| 花生球蛋白基因克隆及其表达分析 |
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| 引用本文： | 全先庆,杨辉霞,单雷,毕玉平.花生球蛋白基因克隆及其表达分析[J].安徽农业科学,2006,34(21):5483-5484,5490. |
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| 作者姓名： | 全先庆 杨辉霞 单雷 毕玉平 |
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| 作者单位： | 山东师范大学生命科学学院,山东济南,250014;临沂师范学院生命科学学院,山东临沂,276005;山东师范大学生命科学学院,山东济南,250014;山东省农业科学院高新技术研究中心,山东济南,250100 |
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| 基金项目： | 山东省自然科学基金项目（Z2002D06）. |
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| 摘 要： | 利用噬菌斑原位杂交技术从花生未成熟种子cDNA文库中筛选到一个花生种子贮藏蛋白基因全长cDNA,该cDNA包含一个1 886 bp的开放阅读框,可编码536个氨基酸残基,Northern分析发现,该基因在种子发育早期开始表达,在之后的各个时期表达水平均较高。
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| 关 键 词： | 花生 球蛋白 cDNA 表达 |
| 文章编号： | 0517-6611（2006）21-5483-02 |
| 收稿时间： | 2006-03-18 |
| 修稿时间： | 2006-03-18 |
Cloning and Expression Analysis of an Arachin Gene from Arachis hypogaea L. |
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| QUAN Xian-qing ,et al.Cloning and Expression Analysis of an Arachin Gene from Arachis hypogaea L.[J].Journal of Anhui Agricultural Sciences,2006,34(21):5483-5484,5490. |
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| Authors: | QUAN Xian-qing |
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| Institution: | College of Life Sciences, Shandong Normal University, Jinan, Shandong 250014 |
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| Abstract: | A full-length cDNA of an arachin gene was cloned from a cDNA library constructed with immature peanut seeds by plaque in situ hybridization.The cDNA contained an ORF of 1?886 bp with a deduced protein of 536 amino acid residues.Results of northern blot analysis showed that the expression of the cloned gene occurred at an early stage of seed development,was increased dramatically in the following stage and retained the higher accumulation of mRNA until mature. |
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| Keywords: | Arachis hypogaea L Arachin cDNA Expression |
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