| PEG-6000模拟干旱及NaCl和温度胁迫对不结球白菜种子活力的影响 |
| |
| 引用本文： | 吴汉花,曹雪,蒋芳玲,吴震.PEG-6000模拟干旱及NaCl和温度胁迫对不结球白菜种子活力的影响[J].种子,2012,31(7):1-6,12. |
| |
| 作者姓名： | 吴汉花 曹雪 蒋芳玲 吴震 |
| |
| 作者单位： | 农业部南方蔬菜遗传改良重点开放实验室,江苏 南京 210095;南京农业大学园艺学院,江苏 南京 210095 |
| |
| 摘 要： | 以不结球白菜品种矮脚黄种子为材料,研究聚乙二醇(PEG-6000)模拟干旱胁迫、NaCl胁迫和温度胁迫下,种子萌发和内部抗氧化相关特性及其渗透调节物质的变化。结果表明:不结球白菜种子的发芽势、发芽率、发芽指数和活力指数随3种胁迫的加重而显著下降,不正常苗率显著上升;随着3种胁迫的加重,SOD和POD活性均下降,CAT活性先有上升的趋势,胁迫加重时降低,活性氧产生速率加快,H_2O_2积累,造成生物膜破坏,相对电导率上升;逆境胁迫下,渗透调节物质可溶性糖、可溶性蛋白和脯氨酸含量显著增加。综上说明,不结球白菜种子在PEG-6000模拟干旱胁迫、NaCl胁迫和温度胁迫下,其种子抗氧化系统代谢失去协调作用的能力,并造成活性氧积累伤害,渗透调节紊乱,从而降低了种子活力直至种子死亡。
|
| 关 键 词： | 不结球白菜 PEG-6000模拟干旱 NaCl胁迫 温度胁迫 种子活力 |
Effect of Vigor Index of Non-heading Chinese Cabbage Seeds under PEG-6000 Simulating Drought, NaCl and Temperature Stress |
| |
| WU Han-hua
,
CAO Xue
,
JIANG Fang-ling
,
WU Zhen.Effect of Vigor Index of Non-heading Chinese Cabbage Seeds under PEG-6000 Simulating Drought, NaCl and Temperature Stress[J].Seed,2012,31(7):1-6,12. |
| |
| Authors: | WU Han-hua CAO Xue JIANG Fang-ling WU Zhen |
| |
| Institution: | 1,2) (1.Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing 210095,China; 2.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China) |
| |
| Abstract: | In this study,using non-heading Chinese cabbage（Brassica campestris ssp.chinensis Makino.） variety Aijiaohuang as material,seed germination,antioxidant-related indexes and osmoregulation substance were studied undre PEG-6000 simulating drought,NaCl and temperature stress.The results showed that: germination energy,germination rate,germination index and vigor index decreased with increasement of the three stresses,but abnormal seedling increased.With increasement of the three stesses,superoxide dismutase （SOD） and peroxidase（POD） activity decreased,catalase（CAT）activity firstly went up and then went down, active oxygen production speed increased,the hydrogen peroxide（H2O2） accumulated,so biofilm was damaged and the relative electrolyte leakage was increased.In the adversity stress,soluble suger,soluble protein and proline content increased.Comprehensive analysis showed that under the PEG-6000 simulating drought,NaCl and temperature stress,the disorder of antioxidant system led to reactive oxygen metabolism imbalance,the ability of osmotic adjustment was disorder,so caused by seed vigor decreased and even death. |
| |
| Keywords: | non-heading chinese cabbage PEG-6000 simulating drought NaCl stress temperature stress seed vigour |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
