Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene |
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| Authors: | Giovani Greigh de Brito Eveline Teixeira Caixeta Ana Paula Gallina Eunize Maciel Zambolim Laércio Zambolim Valdir Diola Marcelo Elhers Loureiro |
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| Institution: | 1.Departamento de Pesquisa desenvolvimento & Inova??o, Laboratório de Biotecnologia Vegetal,Embrapa Algod?o,Campina Grande,Brazil;2.Embrapa Café, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO),Universidade Federal de Vi?osa,Vi?osa,Brazil;3.Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO),Universidade Federal de Vi?osa,Vi?osa,Brazil;4.Departamento de Biologia Vegetal,Universidade Federal de Vi?osa,Vi?osa,Brazil |
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| Abstract: | The most important disease of Coffea arabica is coffee leaf rust caused by the fungus Hemileia vastatrix. The purpose of this study was to characterize the inheritance of coffee resistance gene(s) to race II of this pathogen and
to identify and map molecular markers linked to this trait. Different populations were used: F2 (160 plants), BCr (20), and BCs (135), derived from a cross between the resistant genotype Híbrido de Timor UFV 427-15 and
the susceptible cultivar Catuaí Amarelo UFV 2143-236 (IAC 30). The segregation analysis showed that the resistance of Híbrido
de Timor to race II of the H. vastatrix is conferred by a single dominant gene. The amplification of 176 AFLP (Amplified fragment length polymorphism) primer combinations
using bulked segregant analysis (BSA) allowed the identification of three molecular markers linked to the resistance gene.
Genetic mapping of these three markers in the F2 population indicated that they are distributed on both sides, flanking the resistance gene. The markers E.CTC/M.TTT405 and
E.CGT/M.TGT300 were found linked to the resistance gene at 8.69 cM (LOD 18.91) and 25.10 cM (LOD 5.37), respectively, while
E.CCT/M.TTC230 was localized on the other side of the gene, at 20.50 cM (LOD 6.15). These markers are the first rust resistance
markers identified in Híbrido de Timor and can be useful for marker assisted selection in coffee breeding programs. |
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