| 中途添加不同浓度蔗糖对铁皮石斛原球茎多糖积累的影响 |
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| 引用本文： | 苏江,岑忠用,何铁光.中途添加不同浓度蔗糖对铁皮石斛原球茎多糖积累的影响[J].广东农业科学,2010,37(9):65-67. |
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| 作者姓名： | 苏江 岑忠用 何铁光 |
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| 作者单位： | 1. 河池学院化学与生命科学系,广西,宜州,546300
2. 广西农科院蔬菜研究所,广西,南宁,530007 |
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| 摘 要： | 以铁皮石斛类原球茎为材料,研究了在培养过程中添加不同浓度的蔗糖对铁皮石斛原球茎多糖积累的影响,初步探讨中途补料对石斛原球茎多糖积累及培养液中蔗糖消耗的动态变化过程,结果表明:在培养第20d添加5g/L蔗糖对原球茎多糖积累最为有利,干重量及多糖含量、多糖产量最高,分别为16.02g/L、300mg/g、4805.85mg/L,且在整个培养过程,蔗糖的利用率最高,原球茎的活力最强。
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| 关 键 词： | 铁皮石斛 蔗糖 多糖积累 |
Effects of different sucrose concentration of intermittent feed on accumulation of polysaccharides in Dendrobium Candidum |
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| SU Jiang,CEN Zhong-yong,HE Tie-guang.Effects of different sucrose concentration of intermittent feed on accumulation of polysaccharides in Dendrobium Candidum[J].Guangdong Agricultural Sciences,2010,37(9):65-67. |
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| Authors: | SU Jiang CEN Zhong-yong HE Tie-guang |
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| Institution: | SU Jiang1,CEN Zhong-yong1,HE Tie-guang2 (1.Department of Chemistry and Life Science,Hechi University,Yizhou 546300,China,2.Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China) |
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| Abstract: | Protocormn-like bodies of D. Candidum was used as material, the experiment studied on effects of different sucrose concentration of intermittent feed on accumulation of polysaccharides during the culture times, as well as the dynamic change of accumulation of polysaccharides in D. Candidum and sucrose consumption in liquid culture media were discussed, the results showed that: intermittent feed 5 g/L sucrose after 20 d culturing was benefited to the accumulation of polysaccharides, and the dry weight, conte... |
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| Keywords: | Dendrobium Candidum sucrose accumulation of polysaccharides |
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