| 基于toxR基因病原哈氏弧菌PCR检测方法的建立 |
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| 引用本文： | 姚东瑞,朱明,陈丽,毕可然,史爱琴,张晓君.基于toxR基因病原哈氏弧菌PCR检测方法的建立[J].水产科学,2011,30(9):559-562. |
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| 作者姓名： | 姚东瑞 朱明 陈丽 毕可然 史爱琴 张晓君 |
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| 作者单位： | 淮海工学院海洋学院,江苏省海洋资源开发研究院,江苏连云港222005 |
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| 基金项目： | 江苏省水产三项工程项目(PJ2010-58); 科技部科技人员服务企业行动项目(2009GJC10044); 江苏省自然科学基金资助项目(BK2009163) |
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| 摘 要： | 基于toxR基因序列设计检测哈氏弧菌的1对特异性引物,通过PCR反应条件优化、PCR反应特异性及敏感性检测,建立一种哈氏弧菌的特异性分子检测方法.试验结果表明,该引物可使哈氏弧菌扩增出大小为390 bp的toxR基因片段,3种水产动物病原弧菌未扩增出任何条带,敏感性检测结果为该PCR反应最低能检测出0.375 ng/μ...
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| 关 键 词： | toxR基因 哈氏弧菌 PCR检测 |
Development of PCR Method for Detection of Pathogenic Baterium Vibrio harveyi Based on ToxR Gene |
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| YAO Dong-rui,ZHU Ming,CHEN Li,BI Ke-ran,SHI Ai-qin,ZHANG Xiao-jun.Development of PCR Method for Detection of Pathogenic Baterium Vibrio harveyi Based on ToxR Gene[J].Fisheries Science,2011,30(9):559-562. |
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| Authors: | YAO Dong-rui ZHU Ming CHEN Li BI Ke-ran SHI Ai-qin ZHANG Xiao-jun |
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| Institution: | YAO Dong-rui,ZHU Ming,CHEN Li,BI Ke-ran,SHI Ai-qin,ZHANG Xiao-junCollege of Ocean,Huaihai Institute of Technology,Jiangsu Marine Resources Development Research Institute,Lianyungang 222005,China) |
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| Abstract: | In this study,a specific method for detection of pathogenic bacterium Vibrio harveyi,a serious pathogen in aquaculture industry,was established by designing one pair of specific primers based on the toxR gene sequences,by optimizing the PCR reaction condition,and by studying the specificity and sensitivity.The results showed that the PCR primers amplified 390 bp toxR gene fragment from the chromosomal DNA of V.harveyi,and no positive reaction was detected in 3 other pathogenic Vibrio species tested.The PCR ... |
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| Keywords: | toxR gene Vibrio harveyi PCR detection |
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