|
|||||
|
|
| 离子对高效液相色谱法测定日本囊对虾中喹诺酮类药物残留 | |
| 引用本文： | 刘桂英,宛立,葛坤,王年斌,宋永刚,杨斌.离子对高效液相色谱法测定日本囊对虾中喹诺酮类药物残留[J].水产科学,2011,30(11):698-701. |
| 作者姓名： | 刘桂英 宛立 葛坤 王年斌 宋永刚 杨斌 |
| 作者单位： | 1. 辽宁省海洋水产科学研究院,辽宁 大连,116023 2. 大连海洋大学,辽宁 大连,116023 3. 大连海洋学校,辽宁大连,116023 4. 大连石化公司,辽宁 大连,116031 |
| 摘 要： | 采用离子对反相高效液相色谱法检测日本囊对虾中的喹诺酮类药物残留,使用Zorbax SB-C18柱(150mm×4.6mm,5μm)分离,流动相为乙腈∶水(0.01mol/L四丁基溴化铵溶液)=8∶92,流速为1.0ml/min,荧光检测器检测,激发波长为280nm,发射波长为480nm,柱温25℃。试验结果表明,每一种药物在一定的进样量范围内均有良好的线性关系,相关系数r均大于0.9998(n=6);氧氟沙星、环丙沙星和恩诺沙星的加标回收率均在80%以上,相对标准偏差小于10%。 |
| 关 键 词： | 喹诺酮类 高效液相色谱法 日本囊对虾 测定 |
Detecion of Fluoroquinolone Residues in Kuruma Shrimp Marsupenaeus japonicus by Ion-Pair HPLC |
|
| LIU Gui-ying,WAN Li,GE Kun,WANG Nian-bin,SONG Yong-gang,YANG-bin.Detecion of Fluoroquinolone Residues in Kuruma Shrimp Marsupenaeus japonicus by Ion-Pair HPLC[J].Fisheries Science,2011,30(11):698-701. | |
| Authors: | LIU Gui-ying WAN Li GE Kun WANG Nian-bin SONG Yong-gang YANG-bin |
| Institution: | LIU Gui-ying1,WAN Li 2,GE Kun3,WANG Nian-bin1,SONG Yong-gang1,YANG-bin4(1.Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China,2.Dalian Ocean University,3.Dalian Ocean School,4.Dalian Petrochemical Corporation,PetroChina Dalian 116031,China) |
| Abstract: | Fluoroquinolone residues were analyed in kuruma shrimp Marsupenaeus japonicus by Ion-Pair HPLC in which separation was carried out on Zorbax SB-C18 column(150 mm × 4.6 mm,5 μm),with the mobile phase of CH3CN∶H2O(0.01 mol/L tetrabutyl ammonium bromide)=8∶92,and the flow rate of 1.0 ml/min at 25 ℃.The result showed that three calibration curves exhibited good linear regressions for the fluoroquinolone with correlation coefficient of each more than 0.9998(n=6).The average recovery rate of the fluoroquinolone was found to be above 80%,the RSD of less than 10%. |
| Keywords: | fluoroquinolone HPLC Marsupenaeus japonicus detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |