| 用乙基环丙沙星重建受大肠杆菌污染的BHK-21细胞 |
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| 引用本文： | 高林,廖德芳,姚俊,张念祖,李华春,李志华.用乙基环丙沙星重建受大肠杆菌污染的BHK-21细胞[J].中国畜牧兽医,2011,38(11):214-217. |
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| 作者姓名： | 高林 廖德芳 姚俊 张念祖 李华春 李志华 |
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| 作者单位： | 云南省畜牧兽医科学院 云南省热带亚热带动物病毒病重点实验室 农业部热带亚热带 动物病毒学重点开放实验室,云南昆明 650224 |
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| 基金项目： | 云南省应用基础研究项目(2010ZC228) |
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| 摘 要： | 为重建受大肠杆菌污染的BHK-21细胞,本研究采用药物乙基环丙沙星,先测出该药对大肠杆菌的最低抑制浓度(minimum inhibitory concentration,MIC),再测出该药对BHK-21细胞的MIC,从而确定10 mg/L为乙基环丙沙星不影响细胞生长,又能抑制大肠杆菌的安全浓度。然后用含该浓度乙基环丙沙星的MEM生长液,对受污染的BHK-21细胞进行物理、化学相结合的循环处理。经过3次循环处理,再传3代后,确认大肠杆菌污染已经被彻底清除,细胞恢复了健康。此方法为抢救受细菌污染的细胞提供了参考。
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| 关 键 词： | 乙基环丙沙星 大肠杆菌 BHK-21细胞 清除污染 |
| 收稿时间： | 2011-03-23 |
Reconstruct the Contaminated BHK-21 Cells by Escherichia coli with Enrofloxacin |
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| GAO Lin,LIAO De-fang,YAO Jun,ZHANG Nian-zu,LI Hua-chun,LI Zhi-hua.Reconstruct the Contaminated BHK-21 Cells by Escherichia coli with Enrofloxacin[J].China Animal Husbandry & Veterinary Medicine,2011,38(11):214-217. |
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| Authors: | GAO Lin LIAO De-fang YAO Jun ZHANG Nian-zu LI Hua-chun LI Zhi-hua |
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| Institution: | Key Laboratory of Tropical & Subtropical Animal Virology of the Ministry of Agriculture, Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory,Yunnan Animal Science and Veterinary Institute,Kunming 650224,China |
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| Abstract: | The aim is to reconstruct the BHK-21 cells contaminoted by Escherichia coli using Enrofloxacin.This study tested the MIC of Escherichia coli and BHK-21 cells,found the safe concentration of Enrofloxacin was 10 mg/L,it didn't effect the growth of BHK-21 cells,and could inhibit the Escherichia coli.By physical and chemical method,the contaminated BHK-21 cells were treated with the MEM growing solution of the same concentration of Enrofloxacin circularly.The Escherichia coli were cleaned successfully after 3 c... |
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| Keywords: | Enrofloxacin Escherichia coli BHK-21 cells clean contamination |
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