| 仿刺参体壁再生形态学和组织学的研究 |
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| 引用本文： | 李霞,周海燕,秦艳杰,郭娜,孙毅.仿刺参体壁再生形态学和组织学的研究[J].大连水产学院学报,2011,26(4):287-290. |
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| 作者姓名： | 李霞 周海燕 秦艳杰 郭娜 孙毅 |
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| 作者单位： | 大连海洋大学农业部海水增养殖学重点开放实验室,辽宁大连,116023 |
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| 基金项目： | 国家自然科学基金资助项目(30371099); 辽宁省自然科学基金资助项目(20052139); 辽宁省创新团队项目(2007T015); 辽宁省教育厅重点实验室项目(2008S064) |
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| 摘 要： | 用手术刀片切掉仿刺参体壁表皮层和结缔组织层,深度至体腔膜。术后将仿刺参放入正常海水中饲养,用肉眼和光学显微镜观察仿刺参体壁再生的外部形态和组织学变化,并通过免疫荧光方法检测小鼠抗人角蛋白19（CK19）的表达。形态学观察表明：术后1 d,伤口呈凹陷状,体腔膜上增生出一薄层乳白色结缔组织;术后7 d伤口表面出现色素;术后11 d凹陷基本变平;术后21 d,伤口处体壁形态与颜色恢复至术前。组织学观察表明：术后1 d,疏松结缔组织直接暴露,创伤面附近有成纤维细胞和胶原纤维聚集;术后3 d,结缔组织外层有上皮细胞聚集;术后4 d,结缔组织间可见一条带状间隙;术后7 d,表皮外层出现角质层;术后11 d,上皮层厚度接近正常组织,但结缔组织较松散;术后21 d,上皮细胞数量、排列以及结缔组织的排列同正常组织。免疫荧光检测结果表明：CK19在正常的仿刺参体壁中表达得比较分散;术后3 d,CK19在创伤面附近集中表达。
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| 关 键 词： | 仿刺参 体壁 再生 形态学 组织学 |
Morphology and histology of the regenerated body wall of sea cucumber Apostichopus japonicus |
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| LI Xia,ZHOU Hai-yan,QING Yan-jie,GUO Na,SUN Yi.Morphology and histology of the regenerated body wall of sea cucumber Apostichopus japonicus[J].Journal of Dalian Fisheries University,2011,26(4):287-290. |
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| Authors: | LI Xia ZHOU Hai-yan QING Yan-jie GUO Na SUN Yi |
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| Institution: | LI Xia,ZHOU Hai-yan,QING Yan-jie,GUO Na,SUN Yi(Key Laboratory of Mariculture,Agriculture Ministry,PRC,Dalian Ocean Univ.,Dalian 116023,China) |
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| Abstract: | The morphology and histological changes were observed in the regenerated body walls in sea cucumber Apostichopus japonicus whose epidermis and the connective tissue layer were cut off and reared in the sea water by naked eye and a light microscopy.The CK19 was expressed in the mice by fluorescence radioimmunoassay.A thin milky connective tissue was found in the body cavity membranes and hollowness was visible 1 day after regeneration.Pigment was appeared 7 days after regeneration,and no hollowness 11 days a... |
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| Keywords: | Apostichopus japonicus body wall regeneration morphology histology |
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