Construction of a linkage map and identification of DNA markers linked to Fom-1, a gene conferring resistance to Fusarium oxysporum f.sp. melonis race 2 in melon |
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| Authors: | Takahiro Tezuka Keisuke Waki Kazutoshi Yashiro Maki Kuzuya Tomoko Ishikawa Yasumasa Takatsu and Makoto Miyagi |
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| Institution: | (1) Plant Biotechnology Institute, Ibaraki Agricultural Center, 3165-1 Ago, Kasama Ibaraki, 319-0292, Japan;(2) Present address: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai Osaka, 599-8531, Japan;(3) Chikusei Agricultural Extension Center, Ibaraki Agricultural Center, 615 Nikinari, Chikusei Ibaraki, 308-0841, Japan;(4) Ibaraki Agricultural Academy, Ibaraki Agricultural Center, 4070-186, Nagaoka Ibaraki, 311-3116, Japan |
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| Abstract: | Resistance to Fusarium oxysporum f.sp. melonis race 2 is conferred by a single dominant gene, Fom-1 in melon. Here, we identified DNA markers tightly linked to Fom-1 that could be used for marker assisted selection in breeding programs. First, we developed 125 F2 plants derived from the cross between melon lines P11 (fom-1fom-1) and MR-1 (Fom-1Fom-1). Using the F2 population, we constructed a linkage map including 14 SSR markers which had not been mapped previously. Fom-1 was confirmed to be allocated to linkage group 7. Then, we identified four AFLP markers using bulked segregant analysis.
The AFLP marker TAG/GCC-470 was completely linked to Fom-1 and other three markers were mapped near Fom-1. TAG/GCC-470 and TCG/GGT-400 were respectively converted to STS and CAPS markers. Usefulness of DNA markers was confirmed
in the analysis with several melon cultivars and lines. |
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| Keywords: | Bulked segregant analysis DNA marker Fom-1 Resistance to Fusarium wilt Linkage map Melon |
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