| 钙依赖蛋白激酶基因表达载体的构建及其对拟南芥的遗传转化 |
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| 引用本文： | 段金秀,郭丽,李钢,徐柱文,伏显华,曹致中.钙依赖蛋白激酶基因表达载体的构建及其对拟南芥的遗传转化[J].草业科学,2007,24(3):51-55. |
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| 作者姓名： | 段金秀 郭丽 李钢 徐柱文 伏显华 曹致中 |
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| 作者单位： | 甘肃农业大学草业学院,甘肃,兰州730070;四川川大光耀生物工程有限公司,四川,成都,610063 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 将钙依赖蛋白激酶(CDPK)基因插入到植物表达载体pBI121上,构建成特异性表达载体pBI121-CDPK,并采用农杆菌介导法将其转化进入拟南芥Arabidopsis thaliana Columbia生态型品种中,获得了卡那霉素筛选的抗性植株,通过PCR扩增验证,拟南芥基因组中含有此抗性基因.
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| 关 键 词： | 钙依赖蛋白激酶(CDPK) 表达载体构建 拟南芥 遗传转化 |
| 文章编号： | 1001-0629（2007）03-0051-05 |
| 收稿时间： | 10 21 2005 12:00AM |
| 修稿时间： | 2005-10-21 |
Construction of expression vector containing calcium-dependent protein kinas gene and transformation of the vector into Arabidopsis thaliana |
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| DUAN Jin-Xiu,GUO Li,LI Gang,XU Zhu-wen,FU Xian-hua,CAO Zhi-zhong.Construction of expression vector containing calcium-dependent protein kinas gene and transformation of the vector into Arabidopsis thaliana[J].Pratacultural Science,2007,24(3):51-55. |
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| Authors: | DUAN Jin-Xiu GUO Li LI Gang XU Zhu-wen FU Xian-hua CAO Zhi-zhong |
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| Institution: | 1. Department of Grassland Science,Gansu Agricultural University, Lanzhou 730070, China; 2. Sichuan University Guangyao Bioengineering Ltd. , Chendou 610063, China |
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| Abstract: | A plant expression vector was constructed by inserting calcium-dependent protein kinas gene into pBI121.Calcium-dependent protein kinas gene was transferred into Arabidopsis thaliana mediated by Agrobacterium tumefaciens and under the kanamycin selection pressure.The results of PCR from the kanamycin resistant transformants indicated that calcium-dependent protein kinas gene had been integrated into the A.thaliana. |
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| Keywords: | CDPK(Calcium-Dependent Protein Kinas) expression vector Arabidopsis thaliana transformation |
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