| 天门冬的体外抑菌作用 |
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| 引用本文： | 方芳,张恒,赵玉萍,李文兵,杨磊,李东阳.天门冬的体外抑菌作用[J].湖北农业科学,2012(5):931-934. |
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| 作者姓名： | 方芳 张恒 赵玉萍 李文兵 杨磊 李东阳 |
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| 作者单位： | 淮阴工学院生命科学与化学工程学院 |
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| 基金项目： | 淮阴工学院大学生创新计划项目,淮阴工学院校基金项目(HGC1109);江苏省高等教育教改立项研究重点项目(2011JSJG042) |
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| 摘 要： | 利用水煎法、醇提取法和超声波提取法制备天门冬(Asparagus cochinchinensis(Lour)Merr.)提取液,并通过正交试验对超声波提取工艺进行优化;采用杯碟法测定天门冬提取液的抑菌圈直径;采用液体稀释法测定最低抑菌浓度。结果表明,超声波提取所用条件为70%的乙醇、浸泡时间6 h、提取时间20min和提取温度35℃时,所得天门冬提取液对金黄色葡萄球菌(Staphylococcus aureus)、大肠杆菌(Escherichia coli)和黑曲霉(Aspergillus niger)抑制作用最强,对这3种菌的最低抑菌浓度分别为3.13、6.25和12.50 mg/mL。说明天门冬提取液对金黄色葡萄球菌、大肠杆菌、黑曲霉有较强的抑制作用,是一种天然抑菌剂。
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| 关 键 词： | 天门冬(Asparagus cochinchinensis(Lour)Merr.) 提取工艺 抑菌作用 |
In vitro Antimicrobial Test of Asparagus cochinchinensis(Lour) Merr |
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| FANG Fang,ZHANG Heng,ZHAO Yu-ping,LI Wen-bing,YANG Lei,LI Dong-yang.In vitro Antimicrobial Test of Asparagus cochinchinensis(Lour) Merr[J].Hubei Agricultural Sciences,2012(5):931-934. |
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| Authors: | FANG Fang ZHANG Heng ZHAO Yu-ping LI Wen-bing YANG Lei LI Dong-yang |
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| Institution: | (College of Life Science and Chemical Engineering,Huaiyin Institute of Technology,Huai’an 223003,Jiangsu,China) |
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| Abstract: | Three methods(water decoction,alcohol reflux and ultrasonic extraction) were used to prepare extraction of Asparagus cochinchinensis.And the process of ultrasonic extraction was optimized by orthogonal experiments.Cylinder plate method was used to determine the antimicrobial diameter.The liquid dilution method was used to determine the minimum inhibitory concentration(MIC).Results showed that the optimal extracting technique was extracting for 20 min at 35 ℃ with 70% ethanol after immersing 6 h.Under this condition,the extracts had the most obvious effect on Staphylococcus aureus(the MIC was 3.13 mg/mL),Escherichia coli(the MIC was 6.25 mg/mL) and Aspergillus niger(the MIC was 12.50 mg/mL).The extracts of Asparagus cochinchinensis had obvious effect against S.aureus,E.coli and A.niger.Therefore it is a kind of natural antibacterial agent. |
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| Keywords: | Asparagus cochinchinensis(Lour) Merr extracting technique antimicrobial effect |
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