| 水稻花器介导法转目标基因的研究 |
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| 引用本文： | 李小湘,何迎春,高必达.水稻花器介导法转目标基因的研究[J].中国水稻科学,2003,17(3):196-200. |
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| 作者姓名： | 李小湘 何迎春 高必达 |
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| 作者单位： | 1.湖南省水稻研究所， 湖南 长沙 410125； 2湖南农业大学 植物科技学院， 湖南 长沙 410128 |
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| 摘 要： | 研究和分析了受体品种、质粒浓度对水稻花器介导法转化的影响。供试的20个水稻品种（系）中有9个适用于质粒转化,不同受体品种要求的质粒适宜浓度不同。经潮霉素（hygromycin）筛选、PCR分析、抗稻纹枯病和稻瘟病鉴定、GUS活性检测转基因水稻后代，验证了水稻花器介导法转目标基因的有效性。获得了含细菌几丁质酶基因的优丰162转基因T4 代，它表现出对叶瘟病和纹枯病抗性有明显提高。
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| 关 键 词： | 水稻 转基因 几丁质酶基因 抗病性 |
| 文章编号： | 1001-7216(2003)03-0196-05 |
| 收稿时间： | 1900-01-01; |
Studies on Target Genes Transferred by Rice Floral Organ-Mediated Method |
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| LI Xiao-xiang ,HE Ying-chun ,GAO Bi-da.Studies on Target Genes Transferred by Rice Floral Organ-Mediated Method[J].Chinese Journal of Rice Science,2003,17(3):196-200. |
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| Authors: | LI Xiao-xiang HE Ying-chun GAO Bi-da |
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| Institution: | （;1. Hunan Rice Research Institute; Hunan Academy of Agricultural Sciences; Changsha 410125; China;2.College of Plant Science and Technology; Hunan Agricultural University; Changsha 410128; China） |
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| Abstract: | Effects of receptor varieties, plasmids concentrations on the rice transformation rate via floral organ-mediated method were studied. Of 20 tested rice varieties, nine varieties were suitable for transformation. The feasible plasmids concentrations for rice transformation varied with receptor varieties in a rather wide range of concentrations. The validity of target genes transferred by rice floral organ-mediated method were verified by PCR analysis, evaluation of resistance to sheath blight and blast, screening by hygromycin B and examination of GUS activity in transgenic rice. T 4 transgenic rice plants containing Serratia marcescens chitinase gene showed resistance to rice sheath blight and rice leaf blast after inoculation with pathogens. |
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| Keywords: | rice gene transfer chitinase gene disease resistance |
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