| 山西省不同品种马铃薯凝集素质量分数分析 |
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| 引用本文： | 岳新丽,湛润生,李 建,陈 云,王春珍.山西省不同品种马铃薯凝集素质量分数分析[J].中国农学通报,2019,35(36):38-41. |
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| 作者姓名： | 岳新丽 湛润生 李 建 陈 云 王春珍 |
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| 作者单位： | 1.山西省农业科学院高寒区作物研究所;2.山西大同大学应用生物技术研究所;3.山西省农业科学院果树研究所 |
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| 基金项目： | 山西省农业科学院优势课题组自选项目“马铃薯全粉型种质资源引进筛选及遗传分析”(YYS1701)；山西省农业科学院育种工程项目“抗 病优质高产中晚熟马铃薯新品种选育”(17yzgc078)；山西省现代农业产业技术体系马铃薯产业体系。 |
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| 摘 要： | 为研究山西省不同品种(品系)马铃薯凝集素的质量分数,通过对马铃薯凝集素粗提液进行大孔树脂柱层析法提纯,获得马铃薯凝集素溶液,然后采用测定吸光度与凝集活性效价的方法,评定了山西省大同市不同品种(品系)马铃薯凝集素质量分数高低。结果表明,大孔树脂柱层析法提纯马铃薯凝集素效果良好,滤液可以用于后续的分析测定。根据吸光值和凝血效果综合评定,不同品种(品系)马铃薯凝集素质量分数顺序为：05-32-7＞同薯23号＞晋薯27号＞同薯30号＞晋薯23号。
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| 关 键 词： | 草莓 草莓 炭疽病 生理因子 防御酶 |
| 收稿时间： | 2018/7/24 0:00:00 |
| 修稿时间： | 2019/3/26 0:00:00 |
Potato Lectin of Shanxi Varieties: Analysis on Mass Fraction |
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| Abstract: | To study the mass fraction of potato lectin in different potato varieties (strains) of Shanxi Province, the potato lectin solution was obtained by purifying the potato sample solution by macroporous resin column chromatography. Then, the levels of potato lectin in different potato varieties (strains) in Datong, Shanxi Province were assessed by measuring the absorbance and agglutination activity titer. The results showed that the purification of potato lectin by macroporous resin column chromatography was effective, and the filtrate could be used for subsequent analysis and determination. According to the comprehensive evaluation of absorbance value and coagulation effect, the sequence of potato lectin in different potato varieties (strains) was: 05-32-7> Tongshu 30> Jinshu 27> Tongshu 30> Jinshu 23. |
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| Keywords: | Potato lectin Macroporous resin chromatography Coagulation activity titer |
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