| 香蕉枯萎病菌致病力分化与ISSR遗传多样性分析 |
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| 引用本文： | 黄穗萍,郭堂勋,李其利,唐利华,莫贱友,李焜华,孙宪昀.香蕉枯萎病菌致病力分化与ISSR遗传多样性分析[J].植物保护,2018,44(6):107-114. |
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| 作者姓名： | 黄穗萍 郭堂勋 李其利 唐利华 莫贱友 李焜华 孙宪昀 |
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| 作者单位： | 1. 广西农业科学院植物保护研究所, 广西作物病虫害生物学重点实验室, 南宁 530007; 2. 广西大学农学院, 南宁 530004; 3. 中国科学院微生物研究所, 北京 100101 |
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| 基金项目： | 国家重点研发计划(2016YFC1202100); 广西农业科学院基本科研业务专项(2015YT39); 广西农业科学院科技发展基金(2015JZ40, 2015JZ43); 南宁市青秀区科学研究与技术开发重点研发计划(2017040) |
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| 摘 要： | 香蕉枯萎病是一种破坏香蕉维管束的全株性土传病害。本研究旨在探讨香蕉枯萎病菌致病力分化和遗传多样性。以30株采自我国广西的香蕉枯萎病菌,16株分别来自澳大利亚和我国广东、海南、福建、云南等地的香蕉枯萎病菌为对象,采用伤根灌淋法测定香蕉枯萎病菌的致病力,然后用筛选到的ISSR引物对46个香蕉枯萎病菌菌株和4个对照菌株(3个非致病性尖孢镰刀菌和1个茄腐镰刀菌)进行ISSR遗传多样性分析。结果显示,分离到广西香蕉枯萎病菌1号生理小种(FOC1)8株,致病力强、中、弱类型比例分别为62.5%、12.5%和25%;广西香蕉枯萎病菌4号生理小种(FOC4)22株,致病力强、中、弱类型比例分别为18.18%、63.64%和18.18%。14条ISSR引物扩增出237个条带,多态性条带161个,多态性比例为67.93%,遗传相似系数0.76～0.96。聚类分析显示,以遗传距离0.80为阈值时菌株被分为8个类群,所占比例分别为4%、10%、60%、16%、4%、2%、2%、2%。第三类群全部为香蕉枯萎病菌4号生理小种。第一、二、四和五类群总量的70.59%为香蕉枯萎病菌1号生理小种。第八类群为香蕉枯萎病菌3号生理小种。结果表明,在香蕉枯萎病菌与寄主协同进化中,广西的FOC1和FOC4出现明显致病力分化。1号生理小种的遗传多样性比4号生理小种丰富。广西香蕉枯萎病菌4号生理小种与海南、广东的FOC4遗传相似性较高。香蕉枯萎病菌生理小种类型与遗传多样性相关。致病力变异与遗传多样性无相关性。研究结果对香蕉枯萎病菌种群扩张机制探讨、遗传动态分析以及有效防控措施的制定具有一定的指导意义。
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| 关 键 词： | 香蕉枯萎病 致病力 遗传多样性 |
| 收稿时间： | 2018/1/5 0:00:00 |
| 修稿时间： | 2018/6/20 0:00:00 |
Pathogenicity and ISSR genetic variation analysis of Fusariumoxysporum f.sp. cubense isolates |
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| HUANG Suiping,GUO Tangxun,LI Qili,TANG Lihu,MO Jianyou,LI Kunhu,SUN Xianyun.Pathogenicity and ISSR genetic variation analysis of Fusariumoxysporum f.sp. cubense isolates[J].Plant Protection,2018,44(6):107-114. |
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| Authors: | HUANG Suiping GUO Tangxun LI Qili TANG Lihu MO Jianyou LI Kunhu SUN Xianyun |
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| Institution: | 1. Institute of Plant Protection, Guangxi Academy of Agricultural Sciences, Guangxi Key Laboratory of Biology for CropDiseases and Insect Pests, Nanning 530007, China; 2. College of Agriculture, Guangxi University, Nanning 530004, China; 3. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | Banana wilt is a whole-plant soil-borne disease that destroys banana vascular bundles. This study aimed to evaluate the pathogenicity differentiation and genetic diversity of Fusarium oxysporum f.sp. cubense (FOC). Thirty FOC strains were collected from Guangxi and 16 FOC strains from Australia and Guangdong, Hainan, Fujian, Yunnan of China, and their pathogenicity was tested by root damage irrigation pipe method. The genetic similarity between the 46 FOC strains and four reference strains (three non-pathogenic F. oxysporum and one F. solani) was studied by ISSR genetic diversity analysis using most suitable primers and annealing temperature. The results showed that eight F. oxysporum f.sp. cubense race 1 (FOC1) strains and 22 F. oxysporum f.sp. cubense race 4 (FOC4) strains were obtained from Guangxi. The proportions of strong, medium and weak pathogenic types of FOC1 were 62.5%, 12.5% and 25%, respectively, while the proportions of strong, medium, and weak pathogenic types of FOC4 were 18.18%, 63.64% and 18.18%, respectively. Fourteen ISSR primers produced totally 237 bands and 161 polymorphic bands, with a polymorphic proportion of 67.93%. The genetic similarity coefficient ranged from 0.76 to 0.96. Cluster analysis showed that, when the genetic distance was 0.80, the strains could be divided into eight groups, with a proportion of 4%, 10%, 60%, 10%, 4%, 2%, 2% and 2%, respectively. Among them, all strains of the third group were FOC4, while 70.59% of the total of 1st, 2nd, 4th and 5th groups was FOC1. The 8th group belonged to FOC3. The results indicated that FOC1 and FOC4 of Guangxi had apparent pathogenicity differentiation with coevolution with the host. At the same time, the genetic diversity of FOC1 was richer than that of FOC4. Higher genetic similarities existed between FOC4 of Guangxi and FOC4 of Hainan, Guangdong. It suggested that the types of FOC species were associated with the genetic diversity, but there was no correlation between pathogenicity variation and genetic diversity. These results may help us understand the population expansion mechanism, analyze genetic dynamics and formulate effective management measures against F. oxysporum f.sp. cubense. |
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| Keywords: | Fusarium oxysporum f sp cubense pathogenicity genetic diversity |
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