| 烟草野火病菌hrpZ基因的克隆及蛋白的原核表达 |
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| 引用本文： | 姜兆远,邹晓威,高洁.烟草野火病菌hrpZ基因的克隆及蛋白的原核表达[J].吉林农业大学学报,2009,31(6). |
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| 作者姓名： | 姜兆远 邹晓威 高洁 |
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| 作者单位： | 吉林农业大学农学院,长春,130118 |
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| 基金项目： | 吉林省科技发展计划项目(20060204-2);;农业部转基因专项项目(2008ZX08004-004) |
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| 摘 要： | 采用PCR方法从烟草野火病病原菌Psta218中扩增harpin编码基因hrpZ,将其克隆到原核表达载体pGEX-4T-1上,获得重组表达质粒pGEX-hrpZPsta。将重组质粒pGEX-hrpZPsta转化至大肠杆菌BL21(DE3)菌株,得到重组大肠杆菌BL21/pGEX-hrpZPsta。经IPTG诱导得到14.7 kD的蛋白质。该蛋白质与其他已发现的harpins一样,能够使烟草等植物产生过敏性坏死反应、富含甘氨酸、热稳定以及对蛋白酶K敏感。
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| 关 键 词： | 烟草 野火病菌 hrpZ harpin |
Cloning and Expression of hrpZ Gene from Pseudomonas syringae pv. tabaci |
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| JIANG Zhao-yuan,ZOU Xiao-wei,GAO jie.Cloning and Expression of hrpZ Gene from Pseudomonas syringae pv. tabaci[J].Journal of Jilin Agricultural University,2009,31(6). |
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| Authors: | JIANG Zhao-yuan ZOU Xiao-wei GAO jie |
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| Institution: | JIANG Zhao-yuan,ZOU Xiao-wei,GAO jieCollege of Agronomy,Jilin Agricultural University,Changchun 130118,China |
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| Abstract: | The hrpZ gene was amplified from genome of Pseudomonas syringae pv. Tabaci Psta218 by PCR and then constructed expression vector pGEX-hrpZ_(Psta) with regular molecular cloning operation. The recombinant plasmid was transformed into BL21(DE3) . Recombinant protein was induced by Isopropy-lthio-β-D-Galacgoside (IPTG) . The molecular mass of the fusion protein is 14.7 kD analyzed by SDS -PAGE. The protein, similar to the other known harpins, was heat-stable, which contained abundant glycine(G), but had no cysteine. Furthermore, this protein was sensitive to protease K and able to trigger hypersensitive response (HR) in common tobacco. |
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| Keywords: | tobacco Pseudomonas syringae pv tabaci hrpZ harpin |
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