| 壳聚糖对水稻恶苗病菌和油菜菌核病菌的作用 |
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| 引用本文： | 于汉寿,陈永萱.壳聚糖对水稻恶苗病菌和油菜菌核病菌的作用[J].植物保护学报,2002,29(4):295-299. |
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| 作者姓名： | 于汉寿 陈永萱 |
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| 作者单位： | 南京农业大学资源与环境科学学院微生物学系,南京,210095 |
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| 基金项目： | 江苏省“九五”农业重点攻关项目(BE96316-2) |
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| 摘 要： | 用平板含毒介质法测定了壳聚糖对水稻恶苗病菌(Fusarium moniliforme)和油菜菌核病菌(Sclerotinia sclerotiorum)的影响。结果表明,壳聚糖对F.moniliforme抑制作用相对较强,EC_(50)低于1000mg/L,而对S.sclerotiorum的作用较弱,EC_(50)高于3000mg/L。壳聚糖抑制F.moniliforme菌丝生长,导致菌丝畸形肿胀,产生不正常分支,并且抑制其分生孢子的萌发和芽管伸长。壳聚糖处理后F.moniliforme菌丝细胞膜的透性增加,导致蛋白质渗漏,但对其细胞膜的主要结构成分麦角甾醇的含量影响不明显。在离体培养时,壳聚糖对S.sclerotiorum产草酸毒素无明显影响,但预先经壳聚糖处理后,再接种S.sclerotiorum的油菜叶片中草酸含量显著减少。
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| 关 键 词： | 壳聚糖 油菜菌核病菌 水稻恶苗病菌 |
| 收稿时间： | 4/9/2001 12:00:00 AM |
| 修稿时间： | 2001年4月9日 |
Effects of chitosan on Fusarium moniliforme and Sclerotinia sclerotiorum |
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| Yu Hanshou and Chen Yongxuan.Effects of chitosan on Fusarium moniliforme and Sclerotinia sclerotiorum[J].Acta Phytophylacica Sinica,2002,29(4):295-299. |
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| Authors: | Yu Hanshou and Chen Yongxuan |
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| Institution: | Department of Microbiology, Nanjing Agricultural University, Nanjing 210095, China and Department of Microbiology, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Antifungal activity of chitosan on Fusarivm moniliforme and Sclerotinia sclerotionan was determined on PDA. The results showed that effects of chitosan on F. moniliforme were more than S. sclerotiorum. Chitosan not only increased permeability of plasma membrane of F. moniliforme and induced leakage of cellular proteins of pathogen, but also caused pathogen morphological changes, such as abnormal shapes, swelling and excessive b1 inching, however, it did not affected sterol content in mycelia of F. moniliforme, which is an important structural component of plasma membrane. In vitro , content of oxalic acid produced by S. sclerotiorum did not decrease after treated by chitosan, but it remarkably reduced in rape leaves pretreated by chitosan and inoculated with Sclerotinia sclerotiorum. |
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| Keywords: | chitosan Fusarium moniliforme Sclerotinia sclerotiorum |
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