Paralytic toxins in Taiwanese crab Xanthias lividus |
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| Authors: | Yung-Hsiang TSAI Ping-Ho HO Sen-Shyong JENG AND Deng-Fwu HWANG |
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| Institution: | Department of Food Sanitation, Tajen Institute of Technology, Pingtung;, National Museum of Marine Biology and Aquarium, Checheng, Pingtung, Taiwan;, Department of Food Science, National Taiwan Ocean University, Keelung 202, Taiwan |
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| Abstract: | ABSTRACT: Paralytic toxicity was detected by tetrodotoxin (TTX) bioassay in four and five specimens of new toxic xanthid crab Xanthia lividus collected from Lanyu, south-east Taiwan in April 1999 and Hsiaoliuchiu south-west Taiwan in November 1999, respectively. The average toxicity of crab specimens collected from Lanyu and Hsiaoliuchiu was 230 ± 94 (mean ± SD) mouse units (MU) and 241 ± 114 MU, respectively. The toxin was partially purified by YM-1 membrane ultrafiltration and Bio-Gel P-2 column chromatography. Electrophoresis, high-performance liquid chromatography and gas chromatography–mass spectroscopy analyses showed that the crab toxin was composed mainly of tetrodotoxin (83%), as well as a small amount of gonyautoxin 1-4 (17%). |
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| Keywords: | crab gonyautoxin tetrodotoxin toxicity Xanthia lividus |
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