| 皖西白鹅肝脏中GHR，IGF-1和 IGF-1R基因的表达及与朗德鹅的比较 |
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| 引用本文： | 郑智勇,周杰,朱枫桥,李舜,陆春瑞.皖西白鹅肝脏中GHR，IGF-1和 IGF-1R基因的表达及与朗德鹅的比较[J].农业生物技术学报,2009,17(5):937-938. |
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| 作者姓名： | 郑智勇 周杰 朱枫桥 李舜 陆春瑞 |
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| 作者单位： | 安徽农业大学动物科技学院,合肥,230036 |
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| 摘 要： | 选择90日龄的皖西白鹅和朗德鹅各20只，取肝脏组织。采用半定量逆转录多聚酶链式反应（RT-PCR）的方法，以β-actin为内标，对肝脏组织中生长激素受体（GHR）、胰岛素样生长因子-1（IGF-1）、胰岛素样生长因子-1型受体（IGF-1R）的mRNA表达量分别进行比较分析，并分别与鹅的肝脏重及肝重率进行相关性分析。结果表明：皖西白鹅的肝脏重和肝重率都显著大于朗德鹅（p<0.05）；皖西白鹅肝脏中IGF-1的mRNA表达明显高于朗德鹅（p<0.01）；而GHR、IGF-1R mRNA在两品种间没有显著差异。体重与肝脏重显著正相关（r = 0.35，p<0.05），肝重率与肝脏中IGF-1的mRNA的表达显著正相关（r = 0.67，p<0.01），而与GHR、IGF-1R mRNA没有显著的相关性。结果提示：（1）90日龄的皖西白鹅肝脏生长优于朗德鹅；（2）两品种鹅肝脏生长的差异可能与肝脏中IGF-1 mRNA表达的差异有关。
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| 关 键 词： | 鹅 肝脏 生长激素受体 胰岛素样生长因子-1 胰岛素样生长因子-1型受体 |
| 收稿时间： | 2007-9-17 |
| 修稿时间： | 2008-6-20 |
Comparison of GHR, IGF-1 and IGF-1R expression in Liver between Wanxi White Geese and Landaise Geese |
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| Abstract: | Liver tissue was taken from Wanxi White Geese and Landaise Geese at 90days of age. The number of each breed is 20. The total RNA was extracted from liver to measure the abundance of the growth hormone receptor（GHR）, insulin like growth factor-1（IGF-1）and insulin like growth factor-1 receptor（IGF-1R）by Semi-quantitative RT-PCR using β-actin as an internal standard. Results show that the liver weight and liver index of Wanxi White Geese were significantly higher than in that of Landaise Geese（p<0.05）. IGF-1 mRNA expression was higher in Wanxi White Geese than that in Landaise Geese （p<0.01）. Significant positive correlation was found between body weight and liver weight（r=0.35, p<0.05）. The liver index with the expression of IGF-1mRNA in liver also had significant positive correlation（r=0.67, p<0.01）. The results suggest that, at 90 days of age, (1) The growth of liver was better in Wanxi White Geese than that in Landaise Geese; (2) the higher of liver wight of Wanxi White Geese could be the results of higher expression of IGF-1 mRNA in liver . |
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