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三七内生菌分离及抗根腐病病原真菌筛选
引用本文:张玉洁,李洪超.三七内生菌分离及抗根腐病病原真菌筛选[J].北方园艺,2011(23):130-132.
作者姓名:张玉洁  李洪超
作者单位:文山学院生化系,云南文山663000;文山州生物资源开发研究中心,云南文山663000;麻栗坡县董干镇麻栗堡小学,云南麻栗坡,663609
基金项目:云南省科技厅面上资助项目(YNSF20110303);云南省教育厅重点基金资助项目(2010Z038);文山学院博士启动经费资助项目
摘    要:对三七(Panax notoginseng)内生真菌进行了系统的分离,并以三七根腐病主要病原真菌坏损柱孢菌(Cylindrocarpon destruans)和三七黄腐病菌(Cylindrocarpon did ynum)为对象,对108株三七Panax notoginseng(Burk.)F.H.Chen]内生真菌及其次生代谢产物进行了抑菌活性检测.结果表明:有29株内生菌至少对1种病原菌有抑制作用,14株内生菌对2种病原菌都有抑制作用;21株内生菌的次生代谢产物至少对1种测试菌有抗菌活性,其中的2株对坏损柱孢菌具有很强的抑制作用.说明从三七内生真菌中可以筛选出对三七根腐病主要原真菌具有良好抑制作用的菌种.

关 键 词:三七  内生真菌  根腐病病原真菌  抑菌活性

Isolation of Panax notoginseng Endophytes and Antifugal Activities Agaist Phytopa-thogenic Fungi of Root Rot
ZHANG Yu-jie , LI Hong-chao.Isolation of Panax notoginseng Endophytes and Antifugal Activities Agaist Phytopa-thogenic Fungi of Root Rot[J].Northern Horticulture,2011(23):130-132.
Authors:ZHANG Yu-jie  LI Hong-chao
Institution:ZHANG Yu-jie1,2,LI Hong-chao3(1.Department of Biology,Wenshan University,Wenshan,Yunnan 663000;2.Center for Conservation and Utilization of Bio-resources of Wenshan,Wenshan,Yunnan 663000;3.Elementary School of Malibo Villeage,Malipo,Yunnan 663609)
Abstract:Endophytic fungi was isolated from Panax notoginseng.Antifugal activities of 108 strains of the endophytes and their secondary metabolites agaist phytopathogenic fungi,the C.destruans and C.destruans,was detected.The results showed that 29 strains presented antifungal activities to at least one of the tested fungi,14 strains to two tested fungi;and the second metabolites of 21 strains presented antifungal activities to at least one of the tested fungi,2 strains showed good antifungal activities to C.destruans.This study demonstrated that Antifugal strains toward Root rot pathogen of Panax notoginseng can be find from its endophytes.
Keywords:Panax notoginseng  endophytes  root rot pathogen  antifugal activities
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