| “库拉索”芦荟的组织培养研究 |
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| 引用本文： | 刘思言,姚丹,关淑艳,李胜男,王丕武.“库拉索”芦荟的组织培养研究[J].北方园艺,2011(23):116-118. |
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| 作者姓名： | 刘思言 姚丹 关淑艳 李胜男 王丕武 |
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| 作者单位： | 吉林农业大学生命科学学院,吉林长春,130118;吉林农业大学农学院,吉林长春,130118 |
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| 基金项目： | 转基因生物新品种培育重大专项资助项目(2008ZX08004-004);吉林省科技厅科技发展计划资助项目(201101111) |
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| 摘 要： | 以“库拉索”芦荟为试材,以MS为基本培养基,添加不同浓度的NAA和6-BA,研究不同浓度激素组合对“库拉索”芦荟不定芽诱导及组培苗生根的影响.结果表明:MS+6-BA 4.0mg/L+NAA 0.2 mg/L+AC 500 mg/L为最佳的不定芽诱导培养基；继代培养以MS+6-BA 4.0mg/L+NAA0.1 mg/L+AC 500 mg/L的效果最好；生根培养基以MS+NAA 1.5 mg/L+AC0.12％最佳.
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| 关 键 词： | "库拉索"芦荟 组织培养 不定芽诱导 |
Study on the Tissue Culture of Aloe vera |
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| LIU Si-yan
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YAO Dan
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GUAN Shu-yan
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LI Sheng nan
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WANG Pi-wu.Study on the Tissue Culture of Aloe vera[J].Northern Horticulture,2011(23):116-118. |
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| Authors: | LIU Si-yan YAO Dan GUAN Shu-yan LI Sheng nan WANG Pi-wu |
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| Institution: | LIU Si-yan1,YAO Dan1,GUAN Shu-yan1,LI Sheng-nan1,WANG Pi-wu2(1.College of Life Sciences,Jilin Agricultural University,Changchun,Jilin 130118;2.College of Agriculture,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | Taking Aloe vera as test material,using MS as minimal medium,adding different concentration of NAA and 6-BA,the influence of combination of different concentrations hormone on in vitro shoot induction and tissue seedling root were studied.The results showed that optimal medium for induced adventitious bud was MS+6-BA 4.0 mg/L+NAA 0.2 mg/L+AC 500 mg/L;optimal medium for subculture was MS+6-BA 4.0 mg/L+NAA 0.1 mg/L+AC 500 mg/L;optimal medium for rooting was MS+NAA 1.5 mg/L+AC 0.12%. |
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| Keywords: | Aloe vera tissue culture adventitious bud induce |
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