| 西藏无毛漆姑草的组织培养 |
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| 引用本文： | 杨恒,张辉,孙健,卢珊珊,曾凡景,赵惠恩.西藏无毛漆姑草的组织培养[J].甘肃农业大学学报,2011,46(6):66-72. |
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| 作者姓名： | 杨恒 张辉 孙健 卢珊珊 曾凡景 赵惠恩 |
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| 作者单位： | 北京林业大学园林学院,国家花卉工程技术研究中心,北京 100083 |
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| 摘 要： | 以从西藏藏族自治区引种的无毛漆姑草带腋芽茎段为外植体进行组织培养试验,先后开展了初代培养筛选、继代增殖、生根和移栽等,以筛选出无毛漆姑草适宜的增殖、生根培养基以及移栽基质.结果表明:在丛生芽诱导阶段,细胞分裂素6-BA是主导因子,最适增殖培养基为MS+6-BA0.5 mg/L+NAA0.1 mg/L+30 g/L蔗糖,...
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| 关 键 词： | 无毛漆姑草 组织培养 增殖 生根 移栽 |
Tissue culture of Sagina saginoides from Tibet |
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| YANG Heng,ZHANG Hui,SUN Jian,LU Shan-shan,ZENG Fan-jing,ZHAO Hui-en.Tissue culture of Sagina saginoides from Tibet[J].Journal of Gansu Agricultural University,2011,46(6):66-72. |
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| Authors: | YANG Heng ZHANG Hui SUN Jian LU Shan-shan ZENG Fan-jing ZHAO Hui-en |
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| Abstract: | The tissue culture of Sagina saginoides from Tibet was studied systematically from aspects of disposal,shoot inducement,proliferation and rooting to transplantation.The results showed that in the proliferation period,6-BA was a major factor,the suitable medium was MS+6-BA0.5 mg/L+NAA 0.1 mg/L+30 g/L sugar,in which the propagation coefficient reached to 5.12,and the buds grew well,so the medium was suitable for transplantation.In the rooting period,the rooting rates of medium contained NAA and IBA were all lower than that of only contained IBA,the suitable medium was 1/2 MS+IBA 0.5 mg/L+20 mg/L sugar.In the transplantation period,the survival rate of plantlets with 2.5 mm of root length reached 100% in the mixture soil(leaf mold∶vermiculite as 3∶1). |
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| Keywords: | Sagina saginoides tissue culture proliferation rooting transplantation |
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