| DEB-2菌株抗真菌活性物质对半夏组培生长的影响 |
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| 引用本文： | 吴璇,康冀川,雷帮星,何劲,杨秀超,吴定基.DEB-2菌株抗真菌活性物质对半夏组培生长的影响[J].贵州农业科学,2011(9). |
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| 作者姓名： | 吴璇 康冀川 雷帮星 何劲 杨秀超 吴定基 |
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| 作者单位： | 贵州大学西南药用生物资源教育部工程研究中心;贵州大学生命科学学院;贵州大学贵州省农业生物工程重点实验室; |
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| 基金项目： | 贵州省自然科学基金项目“石斛内生菌对植物病害及组培污染菌的生物防治研究”[黔科合J字(2008)2090]; 贵州省农业攻关项目“辣椒真菌病害生物防治及其田间配套关键技术”[黔科合NY字(2011)3034] |
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| 摘 要： | 为了开发DEB-2菌株抗真菌活性物质成为组培抗真菌添加剂,采用生长速率法研究了DEB-2菌株抗真菌活性物质对12株组培常见污染菌的抑制作用,并将该抗真菌活性物质添加到组培培养基中,观察其对半夏组培苗生长的影响。结果表明,DEB-2菌株抗真菌物质对组培常见污染菌有较强的抑制作用,在20mL/L浓度下能明显抑制大多数常见组培污染真菌的生长,但对半夏的生长无显著影响;在30mL/L浓度下能显著抑制半夏不定芽的再生率;在40mL/L浓度下对不定芽生长高度的抑制作用极显著,对半夏的生长势有明显的抑制作用。
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| 关 键 词： | DEB-2 抗真菌物质 真菌污染 组培 半夏 |
Effects of DEB-2 Antifungal Compound to Tissue Culture of Pinellia ternate |
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| WU Xuan,KANG Ji-chuan,LEI Bang-xing,HE Jin,YANG Xiu-chao,WU Ding-ji.Effects of DEB-2 Antifungal Compound to Tissue Culture of Pinellia ternate[J].Guizhou Agricultural Sciences,2011(9). |
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| Authors: | WU Xuan KANG Ji-chuan LEI Bang-xing HE Jin YANG Xiu-chao WU Ding-ji |
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| Institution: | WU Xuan2,3,KANG Ji-chuan1,2,LEI Bang-xing2,HE Jin1*,YANG Xiu-chao3,WU Ding-ji3(1.Guizhou Key Laboratory of Agricultural Bioengineering,Guizhou University,Guiyang,Guizhou 550025,2.The Engineering and Research Center for Southwest Bio-pharmaceutical Resource of National Ministry of China,3.College of Life Sciences,China) |
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| Abstract: | In order to develop the DEB-2 antifungal compound to become antifungal additive of tissue,the inhibition activity to common contaminated fungi of tissue culture of DEB-2 antifungal compound and the effects of the growth of P.ternate were studied.The results showed that the antifungal compound of DEB-2 had strong inhibition activity to contaminated fungi of tissue culture.At the concentration of 20 mL/L,the antifungal compound could obviously restrain the growth of contaminated fungi,but had no effects to th... |
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| Keywords: | DEB-2 antifungal substance fungal contamination tissue culture Pinellia ternate |
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