| pCAAFP66表达载体的构建及其大豆遗传转化的研究 |
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| 引用本文： | 赵印华,陈颖珊,郭丽琼,覃凤云,林俊芳.pCAAFP66表达载体的构建及其大豆遗传转化的研究[J].大豆科学,2011,30(4):541-545. |
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| 作者姓名： | 赵印华 陈颖珊 郭丽琼 覃凤云 林俊芳 |
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| 作者单位： | 1. 华南农业大学食品学院,广东广州,510642
2. 华南农业大学食品学院,广东广州510642;华南农业大学生物质能研究所,广东广州510642 |
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| 基金项目： | 国家转基因生物新品种培育重大专项资助项目(2009ZX08004-007B); 广东省科技计划资助项目(2009A020102004); 华南农业大学“211工程”三期资助项目(2009C010500001) |
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| 摘 要： | 以pCAMBIA3301为载体骨架,afp为目的基因,构建了大小为9868 bp的大豆表达载体pCAAFP66,该载体带有抗冷冻蛋白基因afp和筛选标记基因bar.以大豆品种华春3号和华春6号的胚尖和子叶节为外植体,应用农杆菌介导法进行遗传转化,以草甘膦(PPT)为筛选底物.结果表明:在胚尖转化体系中,华春3号和华春6...
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| 关 键 词： | 抗冷冻蛋白 载体构建 农杆菌介导 大豆遗传转化 |
Construction of Expression Vector pCAAFP66 and Its Transformation into Soybean by Agrobacterium-mediated Method |
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| ZHAO Yin-hua,CHEN Ying-shan,GUO Li-qiong,QIN Feng-yun,LIN Jun-fang.Construction of Expression Vector pCAAFP66 and Its Transformation into Soybean by Agrobacterium-mediated Method[J].Soybean Science,2011,30(4):541-545. |
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| Authors: | ZHAO Yin-hua CHEN Ying-shan GUO Li-qiong QIN Feng-yun LIN Jun-fang |
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| Institution: | ZHAO Yin-hua1,CHEN Ying-shan1,GUO Li-qiong1,2,QIN Feng-yun1,LIN Jun-fang1,2(1.College of Food Science,South China Agricultural University,Guangzhou 510642,2.Institute of Biomass Research,Guangdong,China) |
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| Abstract: | Plant expression vector pCAAFP66(9868 bp),which contained the target gene afp and the screening marker Bar gene,was constructed for soybean transformation.The anti-freezing gene afp was transformed into embryonic tip and cotyledonary node of soybean using Agrobacterium-mediated method and PPT as selective agent.Identification indicated the afp gene was successfully transformed into Huachun 3 and Huachun 6 through the embryonic tip transformation system,with transformation rates 7.4% and 4.0%,respectively.Wh... |
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| Keywords: | afp gene Vector construction Agrobacterium-mediated Soybean genetic transformation |
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