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乙醇法提取枇杷叶总黄酮的研究
引用本文:张守梅,许奇志,张立杰,胡文舜,张丽梅.乙醇法提取枇杷叶总黄酮的研究[J].福建果树,2011(2):6-10.
作者姓名:张守梅  许奇志  张立杰  胡文舜  张丽梅
作者单位:福建省农业科学院果树研究所,福建省龙眼枇杷育种工程技术研究中心,福建福州350013
基金项目:福建省农业科学院青年科技人才创新基金项目(2006年度)
摘    要:对乙醇法提取枇杷叶黄酮的工艺进行研究,采用单因素试验法对影响枇杷叶中黄酮类物质提取率的主要因素进行了考察,利用正交试验优化了提取黄酮类物质的最佳工艺条件。确定了枇杷叶总黄酮的最佳提取工艺为∶料液比1∶50,提取温度80℃,提取时间3 h,乙醇浓度50%。在最佳提取工艺条件下测得解放钟枇杷叶中总黄酮含量为0.3805%,回收率98.37%。

关 键 词:乙醇法提取  枇杷叶  总黄酮

Research on Loquat Flavonoids Content by Ethanol Extraction
ZHANG Shou-mei XU Qi-zhi ZHANG Li-jie HU Wen-shun ZHANG Li-mei.Research on Loquat Flavonoids Content by Ethanol Extraction[J].Fujian Fruits,2011(2):6-10.
Authors:ZHANG Shou-mei XU Qi-zhi ZHANG Li-jie HU Wen-shun ZHANG Li-mei
Institution:ZHANG Shou-mei XU Qi-zhi ZHANG Li-jie HU Wen-shun ZHANG Li-mei(Fruit Research Institute,Fujian Academy of Agricultural Science,Fujian Fruit Breeding Engineering Technology Research Technology Center for Longan & Loquat,Fuzhou 350013,Fujian China)
Abstract:The technology for extracting flavonoids from loquat leaves was studied with ethanol extraction.The major factors affecting the extraction ratio of flavonoids from loquat leaves were investigated with single factor experiment method,and the optimum process conditions of extracting flavonoids was optimized with orthogonal test method.The identified optimum conditions of extracting flavonoids from loquat leaves are feed liquid mass ratio 1 ∶ 50,extraction temperature 80℃,extraction time 3 h,ethanol 50%.The extraction ratio of flavonoids by this method is 0.3805% and the rate of recovery is 98.37%.
Keywords:Ethanol extraction  Loquat leaves  Flavonoids  
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