| 大豆子叶节高频愈伤组织诱导及其植株再生 |
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| 引用本文： | 孙明杰,魏玄,邹丹丹,赵春梅,蔡春梅,薛仁镐.大豆子叶节高频愈伤组织诱导及其植株再生[J].大豆科学,2011,30(4):546-551. |
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| 作者姓名： | 孙明杰 魏玄 邹丹丹 赵春梅 蔡春梅 薛仁镐 |
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| 作者单位： | 青岛农业大学生命科学学院,山东青岛,266109 |
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| 基金项目： | 国家转基因生物新品种培育科技重大专项资助项目(2008ZX08010-002,2009ZX08010-009B) |
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| 摘 要： | 以萌发7d的大豆子叶节切断及其切片为外植体,研究了萌发培养基、不同激素及其配比对大豆子叶节愈伤组织诱导与分化的影响.结果表明:当不添加BA时,在子叶节切断处几乎无愈伤组织的产生,只有添加BA时,才能产生愈伤组织.外植体切取方式、愈伤组织诱导培养基中BA和TDZ的浓度配比对愈伤组织诱导率产生很大影响.BA浓度为0.05～...
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| 关 键 词： | 大豆 子叶节切断 子叶节切片 愈伤组织 植株再生 |
High Frequency Induction of Callus from Soybean Cotyledonary Node Explants and Its Plant Regeneration |
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| SUN Ming-jie,WEI Xuan,ZOU Dan-dan,ZHAO Chun-mei,CAI Chun-mei,XUE Ren-gao.High Frequency Induction of Callus from Soybean Cotyledonary Node Explants and Its Plant Regeneration[J].Soybean Science,2011,30(4):546-551. |
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| Authors: | SUN Ming-jie WEI Xuan ZOU Dan-dan ZHAO Chun-mei CAI Chun-mei XUE Ren-gao |
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| Institution: | SUN Ming-jie,WEI Xuan,ZOU Dan-dan,ZHAO Chun-mei,CAI Chun-mei,XUE Ren-gao(Department of Life & Science,Qingdao Agricultural University,Qingdao 266109,Shandong,China) |
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| Abstract: | Effects of germination medium and different concentration ratios of BA and TDZ on the callus induction and plant regeneration were studied using fragments and slices of 7 day-germinated soybean cotyledonary nodes as explants.The result showed that the callus formation occurred from the cotyledonary node fragments only when it was cultured on germination medium containing BA.There were significant differences in callus induction frequencies among different explants,different concentration ratios of BA and TD... |
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| Keywords: | Soybean Cotyledonary node fragment Cotyledonary node slice Callus Plant regeneration |
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