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| 鸡IL-18原核表达工程菌遗传稳定性与表达产物的生物学活性检测 | |
| 引用本文： | 赵敏,张春杰,程相朝,李银聚,吴庭才,杨涛.鸡IL-18原核表达工程菌遗传稳定性与表达产物的生物学活性检测[J].河南农业科学,2011,40(2):138-141. |
| 作者姓名： | 赵敏 张春杰 程相朝 李银聚 吴庭才 杨涛 |
| 作者单位： | 1. 河南科技大学,动物科技学院,河南,洛阳,471003;河南农业职业学院,动物科学系,河南,中牟,451450 2. 河南科技大学,动物科技学院,河南,洛阳,471003 3. 河南农业职业学院,动物科学系,河南,中牟,451450 |
| 基金项目： | 河南省自然科学基金项目 |
| 摘 要： | 通过对鸡白介素18(IL-18)原核表达工程菌BL21-ChlL-18遗传稳定性、表达产物的Western-blot及其经Sephadex G-100纯化后的ELISA和淋巴细胞转化试验分析,以检测其遗传稳定性及其表达产物的生物学活性.结果表明,该重组工程菌能稳定地表达鸡IL-18,表达产物不仅能够与抗鸡IL-18的多... |
| 关 键 词： | 鸡 白细胞介素18 原核表达 生物活性 |
Heredity Stability and Bioactivity of Prokaryotic Engineering Bacteria Strain Expressing Chicken IL-18 |
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| ZHAO Min,ZHANG Chun-jie,CHENG Xiang-chao,LI Yin-ju,WU Ting-cai,YANG Tao.Heredity Stability and Bioactivity of Prokaryotic Engineering Bacteria Strain Expressing Chicken IL-18[J].Journal of Henan Agricultural Sciences,2011,40(2):138-141. | |
| Authors: | ZHAO Min ZHANG Chun-jie CHENG Xiang-chao LI Yin-ju WU Ting-cai YANG Tao |
| Institution: | 1.College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China;2.Animal Science Department,Henan Vocational College of Agriculture,Zhongmu 451450,China) |
| Abstract: | In order to detect the heredity stability and bioactivity of prokaryotic engineering bac-teria strain BL21-ChIL-18,its heredity stability and Western-blot analysis of its products were performed and other bioactivity of Sephadex G-100 column purified products were analyzed by ELISA and MTT.The results showed that the recombinant engineering strains can stably express chicken IL-18.Expression products can react with polyclonal antibody anti-chicken IL-18 and induce T lymphocytes proliferation and secreting IFN-γ in vitro.This laid a foundation for further research and the biological function within the chicken body studies. |
| Keywords: | Chicken IL-18 Prokaryotic expression Bioactivity |
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