| 贝类食物中膝沟藻毒素快速筛选方法研究 |
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| 引用本文： | 周秀锦,周向阳,王淑娜,徐君辉,邵宏宏.贝类食物中膝沟藻毒素快速筛选方法研究[J].河南农业科学,2011,40(2):153-156. |
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| 作者姓名： | 周秀锦 周向阳 王淑娜 徐君辉 邵宏宏 |
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| 作者单位： | 舟山出入境检验检疫局,浙江,舟山,316000 |
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| 基金项目： | 浙江省分析测试科技计划项目 |
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| 摘 要： | 为了建立了一种贝类食物中膝沟藻毒素快速筛选方法.将样品用0.05 mol/L的盐酸提取膝沟藻毒素,阴离子柱净化提取液,柱前衍生化反应后在波长为310nm的紫外灯下观察有无荧光产生,快速筛选贝类食物中的膝沟藻毒素.结果表明,衍生化产物在避光条件下30 min内保持稳定,当膝沟藻毒素添加量为0.8 mg/kg时,荧光现象明...
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| 关 键 词： | 贝类食物 膝沟藻毒素 衍生化 快速筛选 |
Development of A Method for Rapid Screening Gonyautoins in Shellfish |
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| ZHOU Xiu-jin,ZHOU Xiang-yang,WANG Shu-na,XU Jun-hui,SHAO Hong-hong.Development of A Method for Rapid Screening Gonyautoins in Shellfish[J].Journal of Henan Agricultural Sciences,2011,40(2):153-156. |
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| Authors: | ZHOU Xiu-jin ZHOU Xiang-yang WANG Shu-na XU Jun-hui SHAO Hong-hong |
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| Institution: | (Zhoushan Entry-exit Inspection and Quarantine Bureau,Zhoushan 316000,China) |
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| Abstract: | A method for rapid screening the gonyautions in shellfish was developed in this study.Gonyautions in the shellfish samples were extracted with 0.05 mol/L hydrochloric acid,the supernatant was cleaned up by passing through SPE anion exchange cartridge and derived by derivatization reagent.The derivatives were inspected by UV lamp at 310 nm.The fluorescence signal of the samples spiked at 0.8 mg/kg with gonyautions were obviously observed and kept stable in 30 minutes.The results showed consistent credibility and accuracy as compared to the method with HPLC.The method of extraction and derivation of gonyautions from shellfish samples were established. |
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| Keywords: | Shellfish Gonyautions Derivatization Rapid screening |
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