| 三元杂交猪IL-18全基因的序列测定及分析(英文) |
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| 引用本文： | 曹素芳,李明,王岩,刘长斗,朱赞梅,唐桂芬,肖松云.三元杂交猪IL-18全基因的序列测定及分析(英文)[J].Agricultural Science&Technology,2009(1). |
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| 作者姓名： | 曹素芳 李明 王岩 刘长斗 朱赞梅 唐桂芬 肖松云 |
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| 作者单位： | 郑州牧业工程高等专科学校;郑州先锋动物药业有限公司;河南省大明动物药业有限公司; |
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| 基金项目： | Supported by Project of Basic and Frontier Technology Research in Henan Province(072300430060 );;Key Scientific and Technological Project of Henan Province(072102130023)~~ |
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| 摘 要： | Objective] To clone the porcine interleukin-18(IL-18) cDNA and explore the immunological effectiveness of porcine IL-18 as an adjuvant of genetic vaccine. Method] The spleen lymphocytes were isolated from Henan three-way cross-breeding pigs. According to the porcine IL-18 gene in GenBank, a pair of specific primers was designed. The full length cDNA of porcine IL-18 was amplified by RT-PCR. Subsequently, porcine IL-18 cDNA was cloned into pGEM-T vector and sequenced and analyzed. Result] The porcine IL-18 gene demonstrated an open reading frame of 579 bp encoding an inactive precursor protein with 192 amino acids. The precursor protein had no typical hydrophobic signal peptide and cleaved by interleukin-1 beta (IL-1β) converting enzyme (ICE) in caspase-1 splice site; the porcine mature protein had biological activity. After comparing with other porcine IL-18 genes, the nucleotide sequence homology was over 96% and the deduced amino acid homology was more than 98%. Conclusion] A full length procine IL-18 gene was gained. It lays the foundation for porcine IL-18 as an adjuvant of genetic vaccine.
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| 关 键 词： | 特异性引物 核苷酸序列 氨基酸序列 基因序列 三元杂交猪 序列测定 同源性 白细胞介素 重组质粒 生物工程 |
Sequencing and Analysis of Porcine Intrleukin-18 Gene |
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| CAO Su-fang,LI Ming,WANG Yan,LIU Chang-dou,ZHU Zan-mei,TANG Gui-fen,XIAO Song-yun.Zhengzhou College of Animal Husb,ry Engineering,Zhengzhou ,.Zhengzhou Xianfeng Animal Pharmaceutical Co.,Ltd,Zhengzhou ,.Henan Daming Animal Pharmaceutical Co.,Zhengzhou.Sequencing and Analysis of Porcine Intrleukin-18 Gene[J].农业科学与技术(英文版),2009(1). |
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| Authors: | CAO Su-fang LI Ming WANG Yan LIU Chang-dou ZHU Zan-mei TANG Gui-fen XIAO Song-yunZhengzhou College of Animal Husb ry Engineering Zhengzhou Zhengzhou Xianfeng Animal Pharmaceutical Co Ltd Zhengzhou Henan Daming Animal Pharmaceutical Co Zhengzhou |
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| Institution: | CAO Su-fang1,LI Ming1,WANG Yan1,LIU Chang-dou2,ZHU Zan-mei3,TANG Gui-fen1,XIAO Song-yun11.Zhengzhou College of Animal Husb,ry Engineering,Zhengzhou 450011,2.Zhengzhou Xianfeng Animal Pharmaceutical Co.,Ltd,Zhengzhou 450032,3.Henan Daming Animal Pharmaceutical Co.,Zhengzhou 450027 |
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| Abstract: | Objective] To clone the porcine interleukin-18(IL-18) cDNA and explore the immunological effectiveness of porcine IL-18 as an adjuvant of genetic vaccine. Method] The spleen lymphocytes were isolated from Henan three-way cross-breeding pigs. According to the porcine IL-18 gene in GenBank, a pair of specific primers was designed. The full length cDNA of porcine IL-18 was amplified by RT-PCR. Subsequently, porcine IL-18 cDNA was cloned into pGEM-T vector and sequenced and analyzed. Result] The porcine IL-1... |
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| Keywords: | Porcine Interleukin-18 Sequencing Homology |
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