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Effects of high and low inspired fractions of oxygen on horse erythrocyte membrane properties, blood viscosity and muscle oxygenation during anaesthesia |
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| Authors: | Karine Portier DMV MSc CertVA PhD David Crouzier† PhD Michel Guichardant‡ PhD Michel Prost§ PhD Jean-Claude Debouzy† MD PhD Nathalie Kirschvink¶ DMV PhD Nicole Fellmann MD PhD Pierre Lekeux†† DMV PhD Diplomate ECVPT & Jean Coudert MD PhD |
| Institution: | Universitéde Lyon,;Lyon, France;Ecole Nationale Vétérinaire de Lyon, Equine Department, Marcy L'Etoile, France Laboratory of biophysics, RMN Group, Centre de Recherche du Service de Santédes Armées, La Tronche, France; University of Lyon: Inserm UMR 870;Insa-Lyon RMND/IMBL;Inra UMR 1235;Villeurbanne, France; SPIRAL, Couternon, France; Laboratory of Animal Physiology, Department of Veterinary Medicine, Faculty of Sciences, University of Namur, Namur, Belgium; CHU de Clermont-Ferrand, Hôpital G. Montpied, Service de Médecine du Sport et des Explorations Fonctionnelles, Clermont-Ferrand, France;INRA, UMR 1019 Clermont-Ferrand, France;UniversitéClermont 1, UMR 1019, UFR Médecine, Clermont-Ferrand, France;CRNH Auvergne, Clermont-Ferrand, France; Physiology, Functional Science Department, Faculty of Veterinary Medicine, University of Liège, Liège, Belgique |
| Abstract: | ObjectivesTo evaluate whether a period of hyperoxia or after a period of hypoxia produced changes attributable to reactive oxygen species in anaesthetized horses.Study designProspective randomized experimental study.AnimalsSix healthy (ASA I) geldings, aged 4.5–9.5 years and weighing 510–640 kg?1.MethodsAfter 30 minutes breathing air as carrier gas for isoflurane, horses were assigned randomly to breathe air as carrier gas (CG0.21) or oxygen as carrier gas (CG1.00) for a further 90 minutes. After an interval of 1 month each horse was re-anaesthetized with the other carrier gas for the 90 minute test period. Ventilation was controlled throughout anaesthesia. Arterial blood was sampled to measure gas tensions, lactate, cholesterol, vitamin E, 4-hydroxy-alkenals, 8-epi-PGF2α, half haemolysis time, half erythrolysis time, and erythrocyte membrane fluidity. Muscle blood flow and oxygenation were evaluated by near infrared spectroscopy and coloured Doppler.ResultsAfter the first 30 minutes horses were hypoxemic. Subsequently the CG1.00 group became hyperoxaemic (PaO2～240 mmHg) whereas the CG0.21 group remained hypoxaemic (PaO2～60 mmHg) and had increased lactate concentration. No significant changes in vitamin E, 4-hydroxy-alkenals, or 8-epi-PGF2α concentrations were detected. During the 90 minute test period the CG0.21 group had increased resistance to free-radical-mediated lysis in erythrocytes, whereas the CG1.00 group had slightly decreased resistance of whole blood to haemolysis. CG0.21 induced a progressive muscle deoxygenation whereas CG1.00 induced an increase in muscle oxygen saturation followed by progressive deoxygenation towards baseline.Conclusions and clinical relevanceDuring isoflurane anaesthesia in horses, the hyperoxia induced by changing from air to oxygen induced minimal damage from reactive oxygen species. Using air as the carrier gas decreased skeletal muscle oxygenation compared with using oxygen. |
| Keywords: | erythrocyte membrane fluidity Horse muscle perfusion and oxygen saturation oxidative stress oxygen inspired fraction |
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