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| 黄瓜老化种子基因组DNA的损伤与修复的RAPD研究 | |
| 引用本文： | 伍春莲,乔爱民,孙敏.黄瓜老化种子基因组DNA的损伤与修复的RAPD研究[J].长江蔬菜,2009(14). |
| 作者姓名： | 伍春莲 乔爱民 孙敏 |
| 作者单位： | 1. 西华师范大学生命科学学院,四川南充,637002 2. 仲恺农业技术学院园艺系 3. 西南大学生命科学学院 |
| 基金项目： | 广东省自然科学基金资助 |
| 摘 要： | 应用RAPD技术对人工老化和PEG处理的黄瓜种子进行扩增多态性DNA分析.建立了黄瓜种子基因组DNA的损伤及其修复的RAPD指纹图谱.在92条引物中有22条可应用于老化种子的研究.16条可应用于PEG处理种子的研究,并从中找出了6条引物S190、S300、S359、S475、S1140、S2144用来比较黄瓜老化种子及其修复的RAPD扩增带型的变化.结果发现,人工老化处理的黄瓜种子的DNA带减少甚至消失.而PEG渗调修复后的黄瓜老化种子DNA带重新出现或者与对照基本相同,从而建立了黄瓜老化种子基因组DNA的损伤与修复的RAPD指纹图谱. |
| 关 键 词： | 黄瓜种子 人工老化 DNA损伤与修复 PEG渗调 |
RAPD Studies on Genomic DNA Damages During Artificial Aging and Its Osmoprimed Repair with PEG in Cucumber Seeds |
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| WU Chunlian,QIA Aimin,SUN Min.RAPD Studies on Genomic DNA Damages During Artificial Aging and Its Osmoprimed Repair with PEG in Cucumber Seeds[J].Journal of Changjiang Vegetables,2009(14). | |
| Authors: | WU Chunlian QIA Aimin SUN Min |
| Institution: | WU Chunlian1,QIAO Aimin2,SUN Min3 ( 1.School of Life Sciences,China West Normal University,Nanchong,Sichuan 637002,2.Departure of Horticulture,Zhongkai Agrotechnical College,3. School of Life Sciences,Southwest University ) |
| Abstract: | RAPD was applied to DNA polymorphism analysis of artificial aged and PEG osmoprimed seeds in Cucumber, and the fingerprints of RAPD were established among genomic DNA damages of cucumber seeds. Twenty-two priemrs from 92 were suitable for cucumber seeds of artificial aging and 16 primers from 92 were suitable for cucumber seeds of PEG treatment. A comparative study was made on DNA bands of cucumber seeds of artificial aging and PEG osmoprimed repairment with six primers S190, S300, S359, S475, S1140 and S21... |
| Keywords: | RAPD Cucumber seed Artificial accelerated aging DNA damage and repair PEG osmopriming RAPD |
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