| 长辣七号辣椒愈伤组织诱导影响因素研究 |
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| 引用本文： | 徐健,刘清波,宋卓,黄红梅.长辣七号辣椒愈伤组织诱导影响因素研究[J].现代农业科技,2012(24):77-79. |
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| 作者姓名： | 徐健 刘清波 宋卓 黄红梅 |
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| 作者单位： | 1. 湖南农业大学东方科技学院,湖南长沙,410128
2. 湖南农业大学生物科学技术学院 |
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| 基金项目： | 湖南农业大学东方科技学院大学生研究性学习与创新性实验计划项目(DFCXY201152) |
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| 摘 要： | 以长辣七号辣椒为材料,以MS为基础培养基,探讨不同外植体、不同激素组合、不同生长日龄子叶、不同培养方式对愈伤组织诱导的影响。结果表明：愈伤组织诱导的最适外植体类型为真叶和子叶;最适的愈伤组织诱导培养基为MS＋6-BA1mg/L＋IAA0.5mg/L;初展期子叶或日龄为10d的子叶,愈伤组织诱导效果最好,诱导率高达100%;在半固体培养方式下的愈伤组织诱导率最高。
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| 关 键 词： | 辣椒 愈伤组织 离体培养 子叶 |
Study on Factors Responsible for Changla 7 Pepper Callus Induction |
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| Authors: | XU Jian LIU Qing-bo SONG Zhuo HUANG Hong-mei |
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| Institution: | 2 *(1 College of Orient Science & Technology,Hunan Agricultural University,Changsha Hunan 410128;2 College of Bioscience and Biotechnology,Hunan Agricultural University) |
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| Abstract: | Using Changla 7 pepper as materials and MS as basic media , the effect of the different explants , different hormone combinations , cotyledons with different days of age and different culturing methods on callus induction were studied. The results showed that the suitable explants for callus induction were euphyllas and cotyledons ; the optimal induction medium was MS+6-BA 1 mg/L+IAA 0.5 mg/L.Callus from cotyledons in early- deployment stages or with 10 days of age grew well and its induction rate was 100%. The callus induction rate was the highest in semisolid culture modes. |
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| Keywords: | pepper callus in vitro culture cotyledons |
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