| 荷兰铁组织培养研究 |
| |
| 引用本文： | 张俊,邱艳红,胡庆,尹斌开.荷兰铁组织培养研究[J].现代农业科技,2012(22):154+158. |
| |
| 作者姓名： | 张俊 邱艳红 胡庆 尹斌开 |
| |
| 作者单位： | 江西省安福县林业局;安福县坳上林场;吉安市森林资源监测中心 |
| |
| 摘 要： | 对荷兰铁组织培养方法进行研究,结果表明:荷兰铁顶芽经消毒处理后接种到MS+6-BA 2.0 mg/L+NAA 0.1 mg/L培养基中,可诱导出愈伤组织和不定芽;在1/2MS+6-BA 1.0 mg/L+NAA 0.1 mg/L培养基中继代培养,扩增倍数(MR值)约2.2倍;将继代培养芽丛中高3cm以上的不定芽接种到生根培养基1/2MS+IAA 0.3 mg/L中,30 d左右生根率达98.2%;炼苗20～30 d后移栽,成活率达95%以上。
|
| 关 键 词： | 荷兰铁 组织培养 技术研究 |
Study on Tissue Culture of Yucca elephantipes |
| |
| Authors: | ZHANG Jun QIU Yan-hong HU Qing YIN Bin-kai |
| |
| Institution: | 1(1 Anfu Forestry Bureau in Jiangxi Province,Anfu Jiangxi 343200;2 Aoshang State Forest Farm in Anfu County; 3 Ji’an Forestry Resource Inspect Center) |
| |
| Abstract: | The tissue culture of Yucca elephantipes was studied.The results showed that the terminal bud of Yucca elephantipes was planted in the culture medium MS+6-BA 2.0 mg/L+NNA 0.1 mg/L after being disinfected,then the callus and adventitious bud were induced.The expansion rate was 2.2 after being subcultured in 1/2MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.Then the subculture buds long than 3 cm were inoculated in rooting medium 1/2MS+IAA 0.3 mg/L,and the rooting rate reached 98.2% after 30 days.The survival rate of transplant was high than 95% after 20～30 days adaptation. |
| |
| Keywords: | Yucca elephantipes tissue culture technology research |
| 本文献已被 CNKI 等数据库收录！ |
