| 与大白菜橘红心基因紧密连锁的SCAR标记 |
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| 引用本文： | 王国臣,张凤兰,余阳俊,张德双,赵岫云.与大白菜橘红心基因紧密连锁的SCAR标记[J].园艺学报,2007,34(1):217-220. |
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| 作者姓名： | 王国臣 张凤兰 余阳俊 张德双 赵岫云 |
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| 作者单位： | (国家蔬菜工程技术研究中心, 北京100097; 首都师范大学生命科学学院, 北京100037) |
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| 基金项目： | 北京市自然科学基金;国家自然科学基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | 利用游离小孢子培养获得的DH系群体和BSA法对与大白菜橘红心基因紧密连锁的分子标记进行了研究, 筛选到与橘红心球色基因or连锁的RAPD标记S1338656和AFLP标记P67M54172 , 其遗传距离分别为8 cM和13 cM, 并将其成功转化成SCAR标记SCOR204和SCOR127。对标记的通用性在110个大白菜自交不亲和系育种材料中进行了验证, 与田间鉴定结果的吻合率分别为90%和89.1%。SCAR标记的获得为大白菜橘红心性状的分子标记辅助选择与基因的图位克隆奠定了基础。
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| 关 键 词： | 大白菜 球叶色 BSA SCAR 标记辅助选择 |
| 文章编号： | 0513-353X（2007）01-0217-04 |
| 收稿时间： | 2006-05-25 |
| 修稿时间： | 2006-05-252006-08-14 |
Identification of SCAR Markers Linked to Orange Head Leaf Gene in Chinese Cabbage(Brassica rapa L. ssp. pekinensis) |
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| WANG Guo-chen,ZHANG Feng-lan,YU Yang-jun,ZHANG De-shuang,ZHAO Xiu-yun.Identification of SCAR Markers Linked to Orange Head Leaf Gene in Chinese Cabbage(Brassica rapa L. ssp. pekinensis)[J].Acta Horticulturae Sinica,2007,34(1):217-220. |
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| Authors: | WANG Guo-chen ZHANG Feng-lan YU Yang-jun ZHANG De-shuang ZHAO Xiu-yun |
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| Institution: | ( ;1. National Engineering Research Center for Vegetables, Beijing 100097, China;2. College of Life Science, CapitalNormal University, Beijing 100037, China) |
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| Abstract: | Bulked segregant analysis was employed to identify molecular markers linked to orange head leaf gene(or)trait based on RAPD and AFLP in Chinese cabbage with a doubled haploid(DH)population derived from the F1 between the two parents 91-112(white head leaf)and T12-19(orange head leaf)via microspore culture.One RAPD marker S1338656 and one AFLP marker P67M54172 were identified to be linked to or gene with the distance of 8 cM and 13 cM respectively.These two markers were successfully transformed into SCAR markers SCOR204 and SCOR127.The accuracy of 90% and 89.1% were obtained when 110 different incompatibility breeding lines of Chinese cabbage were used to be investigated with two SCAR markers,indicating that these two SCAR makers could be used in marker-assisted-selection in orange head leaf breeding programs and positional cloning of or gene in Chinese cabbage. |
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| Keywords: | BSA SCAR |
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