Expression of nucleolus, endosperm storage proteins and disease resistance in an amphiploid between Aegilops tauschii and Secale silvestre |
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| Authors: | Yang Zu-Jun Li Guang-Rong Jiang Hua-Ren Ren Zheng-Long |
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| Institution: | (1) Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan city, 611830 Sichuan, China;(2) Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Yaan city, 625014 Sichuan, China |
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| Abstract: | Cytology and gene expression of an amphiploid between Aegilops tauschiiL., native to China, and Secale silvestre L. were studied to reveal the genomic interaction between the donor species. High frequencies of aneuploids were observed
in the progenies of the amphiploid, indicating its cytological instability. Feulgen staining and Giemsa-C banding showed that
only the nucleolar organizing region from chromosome 5D of Ae. tauschii existed in the amphiploid (2n = 28). The nucleolus of S. silvestre was not observed. Endosperm storage protein electrophoresis indicated most gliadin and glutenin genes from both parents were
expressed in the endosperm of the amphiploid. When inoculated by wheat stripe rust and powdery mildew isolates,the amphiploid
did not express the resistance from its Secale parent,suggesting the presence of disease resistance suppressor(s) in the D genome of Ae. tauschii as well as nucleolar organizer suppressors.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | Aegilops tauschii amphiploid disease resistance nucleolar organizing region Secale silvestre seed storage protein suppressors of resistance |
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