| 河南周口番茄黄化曲叶病病原分子鉴定 |
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| 引用本文： | 胡京昂,刘雪霞,蔡雨惠,曹刚强,应芳卿.河南周口番茄黄化曲叶病病原分子鉴定[J].长江蔬菜,2012(20):83-85. |
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| 作者姓名： | 胡京昂 刘雪霞 蔡雨惠 曹刚强 应芳卿 |
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| 作者单位： | 1. 郑州市蔬菜研究所,450015
2. 郑州大学生物工程系 |
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| 基金项目： | 郑州市普通科技攻关项目（112PPTNYY292）,河南省现代农业大宗蔬菜产业技术体系遗传育种（番茄）岗位专家（S2010-03-02） |
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| 摘 要： | 应用分子生物学的方法鉴定了河南周口地区番茄黄化曲叶病的病原.鉴定结果表明,侵染河南周口番茄的双生病毒为番茄黄化曲叶病毒,与安徽AH-HB1分离物相似性最高,达到98.7%;从构建的系统关系树也可以看出,河南周口的番茄黄化曲叶病毒与安徽AH-HB1分离物的亲缘关系最近,推测河南周口番茄黄化曲叶病毒可能来源于安徽.
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| 关 键 词： | 番茄 番茄黄化曲叶病毒 鉴定 |
Pathogen Identification of Tomato Yellow Leaf Curl Disease in Zhoukou of Henan Province |
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| HU Jing＇ang,LIU Xuexia,CAI Yuhui,CAO Gangqiang,YING Fangqing.Pathogen Identification of Tomato Yellow Leaf Curl Disease in Zhoukou of Henan Province[J].Journal of Changjiang Vegetables,2012(20):83-85. |
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| Authors: | HU Jing＇ang LIU Xuexia CAI Yuhui CAO Gangqiang YING Fangqing |
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| Institution: | 1.Zhengzhou Vegetable Research Institute, 450015; 2.Bioengineenng Department, Zhengzhou University) |
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| Abstract: | In this paper, we identified the pathogen of tomato yellow leaf curl disease in Zhoukou of Henan Province （HN-ZK） by molecular method. The results showed that geminiviruses infecting tomato in Zhoukou was tomato yellow leaf curl virus （TYLCV）, and the obtained sequence from HN-ZK isolate shared the highest similarity （95.7%） with that from Anhui AH-HB1 isolate. It was also indicated that HN-ZK isolate of TYLCV was most closely related to AH-HB1 isolate from the constructed phylogenetic tree. Thus, it was supposed that TYLCV isolate from Zhoukou was probably derived from Anhui. |
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| Keywords: | Tomato Tomato yellow leaf curl virus Identification |
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