| 丛枝菌根真菌对碱胁迫下紫花苜蓿耐碱性的影响 |
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| 引用本文： | 才华,董理,邢易梅,杨圣秋,宋婷婷,许慧慧.丛枝菌根真菌对碱胁迫下紫花苜蓿耐碱性的影响[J].东北农业大学学报,2019,50(10):40-46. |
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| 作者姓名： | 才华 董理 邢易梅 杨圣秋 宋婷婷 许慧慧 |
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| 作者单位： | 东北农业大学生命科学学院,哈尔滨,150030;东北农业大学生命科学学院,哈尔滨,150030;东北农业大学生命科学学院,哈尔滨,150030;东北农业大学生命科学学院,哈尔滨,150030;东北农业大学生命科学学院,哈尔滨,150030;东北农业大学生命科学学院,哈尔滨,150030 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 以紫花苜蓿(Medicago sativa)龙牧806为材料,采用盆栽试验,测定接种摩西球囊霉(Glomus mosseae)后紫花苜蓿生长情况。利用碱胁迫处理5、8 d相对含水量、相对电导率、叶绿素含量、丙二醛(MDA)、抗氧化酶活性、脯氨酸和可溶性糖含量等指标,分析接种丛枝菌根真菌(Arbuscular mycorrhizal fungi, AMF)对提高苜蓿耐碱性的作用。结果表明,正常情况下,接种摩西球囊霉菌苜蓿株高、根长及分支数均高于对照组,叶绿素含量、根中MDA含量,SOD、POD和CAT酶活、脯氨酸含量、可溶性糖含量均高于对照组;碱胁迫后,接种摩西球囊霉可提高紫花苜蓿根和叶中抗氧化酶(POD、SOD和CAT)活性、渗透调节物质(可溶性糖和脯氨酸)含量,降低丙二醛(MDA)含量和细胞膜透性。丛枝菌根真菌摩西球囊霉通过增强紫花苜蓿抗氧化能力,降低碱胁迫对苜蓿造成的损伤,并通过渗透调节能力提高接种苜蓿耐碱性。研究为紫花苜蓿盐碱地种植提供理论依据。
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| 关 键 词： | 丝状菌根真菌(AMF) 摩西球囊霉菌 紫花苜蓿 耐碱性 |
Effect of arbuscular mycorrhizal fungi on alkali tolerance of alfalfa under alkaline stress |
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| Institution: | ,School of Life Sciences, Northeast Agricultural University |
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| Abstract: | Using Medicago sativa cv. Longmu No. 806 as material, a pot experiment was conducted to determine the growth of alfalfa after inoculation with Glomus mosseae. Also, the relative water content, relative conductivity, chlorophyll content, malondialdehyde(MDA), antioxidant enzyme activity, proline and soluble sugar content of alkali stress treatment for 5 and 8 d were measured. These indicators were used to analyze the effects of inoculation of Arbuscular mycorrhizal fungi(AMF) on growth characteristics of alfalfa under alkaline stress. The results showed that under normal conditions,the plant height, root length and number of branches of GI group(G. mosseae inoculated) were higher than those of the NI group(no inoculated), and the contents of chlorophyll and MDA in roots, SOD, POD and CAT activities, proline and soluble sugar contents were also higher than those of the NI group. After alkali stress, inoculation of G.mosseae could increase the activity of antioxidant enzymes(POD, SOD and CAT), the content of osmotic adjustment substances(soluble sugar and proline), and reduce the content of MDA and cell membrane permeability. It concluded that Glomus mosseae could enhance alfalfa antioxidant capacity, reduce the damage of alkali stress of alfalfa, and by improving the osmotic regulation ability improve alkali resistance of alfalfa. This study provided a theoretical basis for the cultivation of alfalfa in saline-alkali soil. |
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| Keywords: | Arbuscular mycorrhizal fungi(AMF) Glomus mosseae alfalfa alkali tolerance |
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