| 油菜菌核病菌菌核室内诱导子囊盘 |
| |
| 引用本文： | 胡宝成 Rimm,SR.油菜菌核病菌菌核室内诱导子囊盘[J].安徽农业科学,1990(2):167-169. |
| |
| 作者姓名： | 胡宝成 Rimm SR |
| |
| 作者单位： | 安徽省农科院作物研究所
(胡宝成)，加拿大马尼托巴大学植物学系(Rimmer S. R.) |
| |
| 摘 要： | 油菜菌核病菌(Sclerotinia sclerotiorum L.) 菌核经过6周以上时间的保湿冷冻处理后,放入温室里,诱导萌发和产生子囊盘.初步试验结果表明:在诱导期间,如果不冲洗,一个月后菌核也不会萌发.隔10天或20天,用灭菌水冲洗一次,冲洗后20天左右,菌核萌发产生子囊盘,并能释放出大量的子囊孢子.隔30天后冲洗,25天后菌核才萌发,但产生异常子囊盘,所释放的子囊孢子数也较少,冷冻处理6周至24周对菌核萌发和子囊盘的产生无显著影响.在室内人工接种时,根据这些结果如何使子囊孢子的释放与油菜盛花期相吻合进行了分析讨论.
|
| 关 键 词： | 油菜 病害 菌核病菌 菌核 子囊盘 诱导 室内条件 人工接种 |
METHOD TO INDUCE APOTHECIA FROM SCLEROTIA OF Sclerotinia Sclerotiorum OF RAPESEED IN GREEN HOUSE |
| |
| Abstract: | The sclerotia of Sclerotinia Sclerotiorum of rapeseed were chilled in
cold room at 4℃ by 6, 12 and 24 weeks, respectively. Then put them
onto Petri dishes with sterilized moist sand to induce germinate in green
house. The results indicated that sclerotia refused to germinate without
wash during inducing time. The sclerotia germinated 20 days later and
produced apothetia with a lot of ascospores after being washed 10 days
or 20 days later at the begining of inducoment. The sclerotia germin-
ated 25 days later and produced abnormal stips and apothecia with less
ascospores after being washed 30 days later at the begining of induc-
ement. There were no significant difference between 6-24 week's chilling
time. How to enable the time that ascospores release from apothecia
coincides with that plants flower among different varieties or different
sowing dates of the same varieties when artificial inoculation using
ascospores conduct in green house was discussed. |
| |
| Keywords: | Rapeseed Sclerotinia sclerotiorum Sclerotia Apothecia Inducement Green house Artificial inoculation |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
