| 不同锌源和锌水平对猪IPEC-J2细胞GLP-2基因表达的影响 |
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| 引用本文： | 计乔平,余冰,韩国全,虞洁,郑萍,何军,毛湘冰,黄志清,陈代文,郭荣富.不同锌源和锌水平对猪IPEC-J2细胞GLP-2基因表达的影响[J].中国畜牧杂志,2013(13):35-38. |
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| 作者姓名： | 计乔平 余冰 韩国全 虞洁 郑萍 何军 毛湘冰 黄志清 陈代文 郭荣富 |
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| 作者单位： | 四川农业大学动物营养研究所,动物抗病营养农业部重点实验室;云南农业大学,云南省动物营养与饲料重点实验室 |
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| 基金项目： | 现代农业产业技术体系建设专项资金资助(CARS-36);教育部长江学者和创新团队发展计划项目资助(IRTO555) |
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| 摘 要： | 本试验旨在探讨不同锌源及锌水平对猪小肠上皮细胞(IPEC-J2)胰高血糖素样肽2(GLP-2)表达的影响。分别以乳酸锌、硫酸锌(锌浓度分别为50、100、150、200 mg/L)作用IPEC-J2细胞,实时荧光定量RT-PCR方法检测GLP-2 mRNA表达,以β-actin mRNA水平作为内参对照。结果表明:在6、12 h检测时间点,不同锌源和水平及其互作对GLP-2 mRNA表达影响极显著(P<0.01);在24 h时不同锌源对其表达影响极显著(P<0.01),锌添加水平对其表达影响显著(P<0.05),不同锌源与锌添加水平交互作用则不显著(P<0.05);乳酸锌、硫酸锌促进GLP-2基因mRNA表达均具有正向浓度效应。乳酸锌和硫酸锌均可上调肠道细胞GLP-2基因mRNA的表达;在同等锌浓度水平下,乳酸锌促进GLP-2基因表达的效果优于硫酸锌。
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| 关 键 词： | 乳酸锌 硫酸锌 IPEC-J2 GLP-2 猪 |
Effects of Different Zinc Sources and Levels on GLP-2 Expressions in IPEC-J2 Cells |
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| JI Qiao-ping,YU Bing,HAN Guo-quan,YU Jie,ZHENG Ping,HE Jun,MAO Xiang-bing,HUANG Zhi-qing,CHEN Dai-wen,GUO Rong-fu.Effects of Different Zinc Sources and Levels on GLP-2 Expressions in IPEC-J2 Cells[J].Chinese Journal of Animal Science,2013(13):35-38. |
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| Authors: | JI Qiao-ping YU Bing HAN Guo-quan YU Jie ZHENG Ping HE Jun MAO Xiang-bing HUANG Zhi-qing CHEN Dai-wen GUO Rong-fu |
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| Institution: | 1.Institute of Animal Nutrition,Sichuan Agricultural University,Animal Disease-Resistance Nutrition Key Laboratory of China Ministry,Sichuan Chengdu 611130,China;2.Yunnan Provincial Animal Nutrition and Feed Key Laboratory,Yunnan Agricultural University,Yunnan Kunming 650201,China) |
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| Abstract: | This experiment approached the effects of zinc sources and levels on GLP-2 expression in swine intestinal epithelium.IPEC-J2 cells were cultured and treated with different concentrations(100,50,150,200 mg/L) of zinc lactate and zinc sulfate.The gene expression of GLP-2 was determined with Real-time RT-PCR using β-action as internal control.The effects of zinc sources,levels and their interaction on GLP-2 expressions in IPEC-J2 cells were significant(P<0.01) as well.Zinc sources significantly improved the expressions of GLP-2 mRNA after 24 h(P<0.01).Zinc concentrations significantly increased GLP-2 expressions in IPEC-J2 cell(P >0.05),however,the interaction between zinc sources and levels was not significant(P >0.05).It was demonstrated that zinc lactate and zinc sulfate both increased the gene expressions of GLP-2 in IPEC-J2 cells,and the effect of zinc lactate on GLP-2 expression was better than zinc sulfate at same concentration. |
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| Keywords: | zinc lactate zinc sulfate IPEC-J2 GLP-2 swine |
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