| 芸芥体细胞胚胎发生的组织细胞学研究 |
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| 引用本文： | 张涛.芸芥体细胞胚胎发生的组织细胞学研究[J].园艺学报,2007,34(1):131-134. |
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| 作者姓名： | 张涛 |
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| 作者单位： | ( 重庆师范大学生命科学学院, 重庆400047; 甘肃省农业科学院, 兰州730070) |
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| 基金项目： | 重庆市自然科学基金;甘肃省自然科学基金;重庆师范大学校科研和教改项目 |
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| 摘 要： | 以芸芥子叶为外植体, 在MS + 2, 4-D 1.0 mg·L-1培养基上培养2周后可诱导体细胞胚胎的发生。胚性愈伤组织在MS + 2, 4-D 0.2 mg·L-1培养基上大量增殖, 体细胞胚胎在N6培养基上成熟后, 可在附加0.1 mg·L-1 IBA的1/2MS培养基上生长。切片观察表明: 芸芥体细胞胚胎为单细胞发生方式, 与合子胚形成过程相似。芸芥胚性细胞具有细胞小、胞质浓、核大、核仁明显, 排列紧密的特点, 首先由单个细胞分裂形成2 - 细胞原胚, 2 - 细胞原胚分裂成3 - 细胞原胚或4 - 细胞原胚, 3 - 细胞原胚或4 - 细胞原胚继续分裂形成多细胞原胚, 进一步发育成一个成熟的体细胞胚胎。
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| 关 键 词： | 芸芥 体细胞胚胎 组织细胞学 |
| 文章编号： | 0513-353X（2007）01-0131-04 |
| 收稿时间： | 2006-08-31 |
| 修稿时间： | 2006-08-312006-12-06 |
Histological Studies of Somatic Embryogenesis in Eruca sativa Mill. |
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| ZHANG Tao.Histological Studies of Somatic Embryogenesis in Eruca sativa Mill.[J].Acta Horticulturae Sinica,2007,34(1):131-134. |
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| Authors: | ZHANG Tao |
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| Institution: | (;1. College of Life Sciences, Chongqing Normal University, Chongqing 400047, China;2.Gansu Academy of Agricultural Sciences,Lanzhou 730070, China) |
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| Abstract: | Somatic embryos were induced from cotyledon explants cultured on Murashige and Skoog(MS) medium supplemented with 1.0 mg·L-1 2, 4-D after two weeks of culture. Embryogenic callus proliferated p rofusely on MS medium containing 0.2 mg·L-1 2, 4-D. Somatic embryosmatured on N6 medium anddeveloped into p lantlets by culturing the mature somatic embryos on 1/2 MS medium containing 0.1 mg·L-1indole-3-butyric acid. Section and microscop ic observation of different developmental stages revealed that somatic embryogenesis was similar to that of the zygotic embryo. It originated from a single embryogenic cell,which had bigger nuclei, thick cytop lasm, clear nucleoli and denser order. The embryogenic cell first dividedinto two-celled stage proembryo, then three- or four-celled stage proembryo. Three- or four-celled stage proembryo developed into multicellular proembryo, then developed into mature somatic embryo. |
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| Keywords: | Eruca sativa Somatic embryos Histological |
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