| 银杏细胞微管免疫荧光观察体系的优化 |
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| 引用本文： | 孙宇涵,杨妮娜,袁存权,胡瑞阳,李云,王谦.银杏细胞微管免疫荧光观察体系的优化[J].东北林业大学学报,2011(9):85-89. |
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| 作者姓名： | 孙宇涵 杨妮娜 袁存权 胡瑞阳 李云 王谦 |
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| 作者单位： | 林木育种国家工程实验室(北京林业大学);北京林业大学 |
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| 基金项目： | 国家自然科学基金项目(3047141230771747) |
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| 摘 要： | 以银杏(Ginkgo biloba L.)雄株为材料,对银杏小孢子母细胞微管的免疫荧光观察体系进行了优化,并以优化后的方法对其减数分裂时期的微管骨架变化情况进行了研究.研究发现:利用PEG包埋可将银杏样本保存1 a以上,经蛋清粘合制片可获细胞数较多的样片,且无需酶解处理即可观察到清晰、完整的银杏小孢子母细胞微管结构.此...
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| 关 键 词： | 银杏 小孢子母细胞 微管 免疫荧光 减数分裂 |
Optimization of Immunofluorescence Observation System for Microtubule in Ginkgo biloba Cell |
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| Sun Yuhan,Yang Nina,Yuan Cunquan,Hu Ruiyang,Li YunNational Engineering Laboratory for Tree BreedingBeijing Forestry University,Beijing,P.R.China;Wang Qian.Optimization of Immunofluorescence Observation System for Microtubule in Ginkgo biloba Cell[J].Journal of Northeast Forestry University,2011(9):85-89. |
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| Authors: | Sun Yuhan Yang Nina Yuan Cunquan Hu Ruiyang Li YunNational Engineering Laboratory for Tree BreedingBeijing Forestry University Beijing PRChina;Wang Qian |
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| Institution: | Sun Yuhan,Yang Nina,Yuan Cunquan,Hu Ruiyang,Li Yun(National Engineering Laboratory for Tree Breeding(Beijing Forestry University),Beijing 100083,P.R.China);Wang Qian(Beijing Forestry University) |
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| Abstract: | Male germ cells of Ginkgo biloba L.were used as the materials to optimize the immunofluorescence observation system of microtubule in G.biloba,and the change of microtubule was preliminarily studied by the optimized observation method during the microsporogenesis of G.biloba.Result showed that the samples of G.biloba could be preserved more than one year by PEG embedding,and the sample plates of more cells could be obtained by albumen bonding.Besides,a clear and intact structure of microtubule cytoskeleton of G.biloba could be observed without enzymolysis treatment.The application of the optimized observation method could prolong the storage life of the immunofluorescence samples,simplify the operating procedure of immunofluorescence observation,and reduce the phenomena of the disorder distribution of cells and superimpose caused by the traditional squash method.Thus,it provides a convenient means for future related work. |
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| Keywords: | Ginkgo biloba Microsporocytes Microtubule Immunofluorescence Meiosis |
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