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| 反相HPLC测定猪血浆中隐丹参酮及其活性代谢物的血药浓度 | |
| 引用本文： | 崔颖,薛明.反相HPLC测定猪血浆中隐丹参酮及其活性代谢物的血药浓度[J].中国兽药杂志,1998,32(4):16-19. |
| 作者姓名： | 崔颖 薛明 |
| 作者单位： | 中国农业科学院兰州畜牧与兽药研究所 |
| 摘 要： | 本试验建立了用反相ＨＰＬＣ同时进行猪血浆中隐丹参酮（ＣＴ）及其活性代谢物丹参酮ⅡＡ（Ｔｓ－ⅡＡ）的快速定量检测方法。采用ＹＷＧＣ１８键合相柱作为分析柱，甲醇－水（８５１５）为流动相，检测波长２５４ｎｍ，二苄基为内标。平均回收率分别为ＣＴ：８８．０９％，Ｔｓ－ⅡＡ：８８．５１％；原药及代谢物的检测限为１０ｎｇ，血浆中最低检出浓度为１６ｎｇ／ｍｌ。日内、日间的变异系数为１．５４％～６．２８％，血浆浓度０．０４～２．５μｇ／ｍｌ范围内呈线性关系，ｒ＝０．９９１４（ＣＴ）及０．９９８８（Ｔｓ－ⅡＡ）。用此法对５头猪进行了ｉｖＣＴ后的ＣＴ及Ｔｓ－ⅡＡ的血浆浓度测定，得出了药时曲线数据。 |
| 关 键 词： | 隐丹参酮 丹参酮ⅡA HPLC |
RP High Performance liquid Chromatographic Determination of Cryptotanshinone and its Active Metabolite in Porcine Plasma |
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| CUI Ying,XUE Ming,LUO Yongjiang,HU Zhenying,ZHANG BIn,ZHOU Zongtian,SHI Yanbin,XIA Wenjiang Lanzhou Institute of Animal and Pharmaceuticals. Chinese Academy of Agricultural Sciences,Key Laboratory of New Animal Medicince Project,Ministy of A.RP High Performance liquid Chromatographic Determination of Cryptotanshinone and its Active Metabolite in Porcine Plasma[J].Chinese Journal of Veterinary Drug,1998,32(4):16-19. | |
| Authors: | CUI Ying XUE Ming LUO Yongjiang HU Zhenying ZHANG BIn ZHOU Zongtian SHI Yanbin XIA Wenjiang Lanzhou Institute of Animal and Pharmaceuticals Chinese Academy of Agricultural Sciences Key Laboratory of New Animal Medicince Project Ministy of A |
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