| 色木槭组织培养技术研究 |
| |
| 引用本文： | 李蕴峰,柴汝松,刘宇明.色木槭组织培养技术研究[J].防护林科技,2013(12):28-29. |
| |
| 作者姓名： | 李蕴峰 柴汝松 刘宇明 |
| |
| 作者单位： | 黑龙江省森林植物园,黑龙江哈尔滨150040 |
| |
| 摘 要： | 以色木槭带芽茎段为外植体进行色木槭组织培养技术研究，通过对不同植物生长调节剂组合的筛选，诱导出丛生芽，获得了再生植株。结果表明：色木槭茎芽最佳灭菌效果是用0．2％ HgCl2灭菌时间12 min ，存活率为65％；茎芽在MS培养基上添加0.2 mg ·L -1 NAA作为启动培养基，添加3．0 mg ·L -16-BA时可以实现增殖，增殖系数最大，为5．0；以1/2MS为基本培养基，取培养后健壮的芽苗，接种到含有0．1 mg ·L -1 NAA的培养基上进行生根培养，建立色木槭的组培体系。
|
| 关 键 词： | 色木槭 植株再生 组织培养 |
Tissue Culture Technology of Acer mono |
| |
| Li Yunfeng,Chai Rusong,Liu Yuming.Tissue Culture Technology of Acer mono[J].Protection Forest Science and Technology,2013(12):28-29. |
| |
| Authors: | Li Yunfeng Chai Rusong Liu Yuming |
| |
| Institution: | (Forest Botanical Garden of Heilongjiang Province, Harbin 150040,China) |
| |
| Abstract: | Tissue culture of Acer mono was conducted by taking Acer mono stems with buds as explants .Different combinations of plant growth regulators were selected ;buds were induced ,and regenerated plants were obtained .Re-sult shows that ：the optimal sterilization effect of Acer mono shoots is 0 .2% HgCl2 ;sterilization time are 12 min;the survival rate are 65% ;adding 0 .2 mg·L-1 NAA shoots on MS medium can be used as boot medium ;adding 3.0 mg·L-1 6-BA can achieve proliferation ;proliferation coefficient are the most largest ,being 5 .0 .Taking 1/2MS as basic medium ,cultured robust shoots were chosen to inoculate 0 .1 mg·L-1 NAA on rooting medium for rooting ＆culture ,and tissue culture system of Acer mono were established . |
| |
| Keywords: | Acer mono regeneration tissue culture |
| 本文献已被 CNKI 维普 等数据库收录！ |
