| 青钱柳叶片、愈伤组织及悬浮细胞中齐墩果酸和熊果酸测定 |
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| 引用本文： | 张月红,上官新晨,尹忠平,陈继光.青钱柳叶片、愈伤组织及悬浮细胞中齐墩果酸和熊果酸测定[J].江西农业大学学报,2012,34(2):363-368. |
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| 作者姓名： | 张月红 上官新晨 尹忠平 陈继光 |
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| 作者单位： | 江西农业大学食品科学与工程学院,江西省高校天然产物研究与开发重点实验室,江西南昌330045 |
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| 基金项目： | 江西省主要学科学术和技术带头人项目，江西省科技厅支撑项目 |
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| 摘 要： | 建立高效液相色谱(HPLC)法测定青钱柳叶片、愈伤组织及悬浮细胞中齐墩果酸和熊果酸含量。考察齐墩果酸和熊果酸在不同色谱柱及流动相下的分离效果,并进行比较分析,确定色谱条件为:色谱柱Waters Sym-mitry C18(4.6 mm×250 mm,5μm),流动相甲醇-0.1%磷酸溶液(87∶13,V/V),流速0.85 mL/min,检测波长210 nm,柱温40℃。结果表明,齐墩果酸和熊果酸标品进样量在0.1～20μg时线性关系良好,相关系数R2均大于0.999,平均加样回收率分别为97.87%、96.91%,RSD为1.90%、2.08%,青钱柳叶片、愈伤组织、悬浮细胞中齐墩果酸、熊果酸的含量差异较大。该方法简便快速,准确可靠,可用于青钱柳齐墩果酸和熊果酸含量的测定。
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| 关 键 词： | 青钱柳 悬浮细胞 愈伤组织 齐墩果酸 熊果酸 |
Determination of Oleanolic Acid and Ursolic Acid in Leaf, Callus and Suspension Cultured Cell of Cyclocarya paliurus |
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| ZHANG Yue-hong
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SHANGGUAN Xin-chen
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YIN Zhong-ping
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CHEN Ji-guang.Determination of Oleanolic Acid and Ursolic Acid in Leaf, Callus and Suspension Cultured Cell of Cyclocarya paliurus[J].Acta Agriculturae Universitis Jiangxiensis,2012,34(2):363-368. |
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| Authors: | ZHANG Yue-hong SHANGGUAN Xin-chen YIN Zhong-ping CHEN Ji-guang |
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| Institution: | (Key Laboratory of Natural Product Research and Development,College of Food Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China) |
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| Abstract: | HPLC method was established for the determination of oleanolic acid and ursolic acid in leaf of Cyclocarya paliurus,callus and suspension cultured cell.The chromatography method was used and the suitable conditions were determined through comparative analysis on the separation performance of oleanolic acid and ursolic acid at different C18 columns and mobile phases.The samples were separated by Waters C18 column(250 mm×4.6 mm,5 μm) at 0.85 mL/min velocity of the mobile phase which consisted of methanol and 0.1% phosphoric acid(87∶13,V/V).The detective wavelength was set at 210 nm and the column temperature was 40 ℃.The calibration curves of oleanolic acid and ursolic acid exhibited good linearity over a concentration range from 0.1 to 20 μg,with correlation coefficients above 0.999.The average recoveries of oleanolic acid and ursolic acid were 97.87% and 96.91% respectively,RSD were1.90% and 2.08%.The contents of oleanolic acid and ursolic acid in leaf,callus and suspension cultured cell of Cyclocarya paliurus were of large difference.This method was simple,accurate and stable,and thus was fit for determining the contents of oleanolic acid and ursolic acid in Cyclocarya paliurus. |
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| Keywords: | Cyclocarya paliurus suspension cultured cell callus oleanolic acid ursolic acid |
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