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| 猪瘟病毒E0蛋白对IFN-β启动子的抑制作用研究 | |
| 引用本文： | 夏燕华,赵天生,陈柳,张楚瑜.猪瘟病毒E0蛋白对IFN-β启动子的抑制作用研究[J].江西农业大学学报,2012,34(3):541-545. |
| 作者姓名： | 夏燕华 赵天生 陈柳 张楚瑜 |
| 作者单位： | 1. 南昌大学医学院,江西南昌,330006 2. 浙江省农业科学院病毒学与生物技术研究所,浙江杭州,310021 3. 武汉大学生命科学学院,湖北武汉,430072 |
| 基金项目： | 江西省自然科学基金资助项目 |
| 摘 要： | 以新城疫病毒(Newcastle disease virus,NDV)为IFN-β诱导剂,将猪瘟病毒(Classical swine fever vires,CSFV)E0基因的C末端与增强型绿色荧光蛋白(Enhanced green fluorescent protem,EGFP)基因相连,瞬时表达于猪。肾细胞PK15中,利用荧光素酶报告基因系统检测IFN-β启动子的活化状态,结果显示CSFV E0蛋白可抑制NDV对IFN-β启动子的诱导作用,抑制效应随E0蛋白量增加而增强;半定量RT-PCR结果显示,E0蛋白可明显降低IFN-βmRNA的生成量。结果表明,E0蛋白具有干扰素拮抗功能。该研究为阐明CSFV的致病及持续感染机制提供了理论支持。 |
| 关 键 词： | 猪瘟病毒 E0蛋白 IFN-β |
A Study of CSFV E0 Protein Block Interferon-beta Induction Mediated by Newcastle Disease Virus (NDV) |
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| XIA Yan-hua , ZHAO Tian-sheng , CHEN Liu , ZHANG Chu-yu.A Study of CSFV E0 Protein Block Interferon-beta Induction Mediated by Newcastle Disease Virus (NDV)[J].Acta Agriculturae Universitis Jiangxiensis,2012,34(3):541-545. | |
| Authors: | XIA Yan-hua ZHAO Tian-sheng CHEN Liu ZHANG Chu-yu |
| Institution: | 1.Medical College,Nanchang University,Nanchang 330006,China;2.Institute of Virology & Biotechnology,Zhejiang Academy of Agriculture Sciences,Hangzhou 310021,China;3.College of Life Science, Wuhan University,Wuhan 430072,China) |
| Abstract: | Newcastle disease virus(NDV) as IFN-P inducer,E0 of classical swine fever virus(CSFV) fused to the enhanced green fluorescent protein(EGFP) was transiently expressed in porcine kidney 15(PK15) cells.In the luciferase activity assay,E0-EGFP was found to block the induction of IFN-P promoter mediated by NDV in a dose-dependent manner.Through IFN-specific semi-quantitative RT-PCR detection,obvious decrease of IFN-P mRNA in NDV-infected PK15 cells was observed in the presence of E0-EGFP.These evidences establish a novel function for CSFV E0 glycoprotein in counteraction of the IFN-P induction pathway. |
| Keywords: | classical swine fever virus E0 protein IFN-β |
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