Detecting and estimating segregation distortion and linkage between glufosinate tolerance and blackleg resistance in Brassica napus L. |
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| Authors: | Rong-Cai Yang Mohan R Thiagarajah Vipan K Bansal Gary R Stringam M Habibur Rahman |
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| Institution: | (1) Alberta Agriculture, Food and Rural Development, Room 300, 7000 – 113 Street, Edmonton, AB, Canada, T6H 5T6;(2) Department of Agricultural, Food and Nutritional Science, University of Alberta, 410 Agriculture/Forestry Centre, Edmonton, AB, Canada, T6G 2P5 |
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| Abstract: | Summary The segregation and linkage between glufosinate (transgenes ‘Rf3’ and ‘T177’) and blackleg resistance genes in canola (Brassica napus L.) were assessed using F1 microspore-derived doubled haploid (DH) populations from four crosses including reciprocals, two involving the transgene ‘Rf3’ and the other two involving the transgene ‘T177’. To relax the assumption of no segregation distortion required for the conventional analysis of segregation and linkage, we employed Bailey's analysis that allows detecting segregation distortion at linked loci. The significant departures from the 1:1 segregation were detected in the crosses involving the transgene ‘T177’ but not in the crosses involving the transgene ‘Rf3’. The apparent deficit of the herbicide tolerant DH lines in the crosses with the transgene ‘T177’ is likely due to differential selection against the gametes carrying ‘T177’ during microspore culture. The linkage was strong between blackleg resistance and the transgene ‘Rf3’ but weak or absent between blackleg resistance and the transgene ‘T177’, suggesting that the two transgenes are probably inserted into distant regions of the genome. The observed linkage offers an opportunity to develop new canola cultivars with both glufosinate tolerance conferred by transgene ‘Rf3’ and blackleg resistance. |
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| Keywords: | Brassica napus Canola doubled haploids linkage test and estimation segregation distortion transgenes |
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