| 云南野生兰属主要种间亲缘关系的AFLP分析 |
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| 引用本文： | 张俊祥,李枝林,范成明,程少丽,赵明富,何月秋.云南野生兰属主要种间亲缘关系的AFLP分析[J].园艺学报,2006,33(5):1141-1144. |
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| 作者姓名： | 张俊祥 李枝林 范成明 程少丽 赵明富 何月秋 |
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| 作者单位： | (云南农业大学植物保护学院, 云南昆明650201; 云南农业大学园林园艺学院, 云南昆明650201) |
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| 基金项目： | 国家自然科学基金项目(30160074)，云南省科学基金重点项目(2002C0003P) |
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| 摘 要： | 采用AFLP技术对云南省兰属的14个种和3个变种中的38份样本进行了亲缘关系分析。16对AFLP引物扩增的条带聚类结果表明, 云南省国兰遗传多样性非常广泛, 相同品种间的不同个体扩增出的条带模式相差很大。依据AFLP数据, 在相似系数0.58的水平下, 38个兰花样品分成3组: 莲瓣兰、春剑、惠兰、寒兰分为第1组, 建兰、豆瓣兰、春兰, 墨兰、虎头兰、西藏虎头兰、大雪兰、碧玉兰、文山红柱兰、长叶兰和兔耳兰分为第2组, 送春和蜜蜂兰分为第3组。
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| 关 键 词： | 兰属 AFLP 遗传多样性 亲缘关系 |
| 文章编号： | 0513-353X（2006）05-1141-04 |
| 收稿时间： | 2005-11-18 |
| 修稿时间： | 2005-11-182006-05-29 |
Phylogenetics Analysis of the Chinese Orchids in Yunnan Province Using AFLP Technique |
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| Zhang Junxiang,Li Zhilin,Fan Chengming,Cheng Shaoli,Zhao Mingfu,He Yueqiu.Phylogenetics Analysis of the Chinese Orchids in Yunnan Province Using AFLP Technique[J].Acta Horticulturae Sinica,2006,33(5):1141-1144. |
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| Authors: | Zhang Junxiang Li Zhilin Fan Chengming Cheng Shaoli Zhao Mingfu He Yueqiu |
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| Institution: | (College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201, China; College of Gardening and Horticulture, Yunnan Agricultural University, Kunming, Yunnan 650201, China) |
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| Abstract: | Thirty-eight collections of Chinese orchids in Yunnan Province, China, including 14 species and 3 varieties, were analyzed phylogenetically using AFLP technique. PCR amplification band pattern with 16 pairs of primers indicated that the Chinese orchids were very diverse, and individuals of the same species had different band patterns. Subgenus Jensoa appeared as a paraphyletic group. Subgenus Cyperorchis was not a monophyletic group instead formed an offshoot of subgenus Jensoa. At a similarity coefficient of 0.58,the orchids could be clustered into 3 groups: Cybidium tortisepalum Wu et Chen, C. tortisepalum var.longibracteatum, C.faberi and C. kancan belonged to Group 1; C. ensifolium, C. goeringii var.serratum, C. goeringii, C. sinense, C.hookerianum, C.tracyanum,C.mastersii,C.lowianum,C.wenshanense,C.erythraeum and C.lancifolium to Group 2; C.cyperifolium var. szechuanicum and C. pumilum to Group 3. |
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| Keywords: | Cybidium AFLP Genetic diversity Phylogenetic relationship |
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