| RP-HPLC测定藏药六味能消胶囊中土木香内酯和异土木香内酯的含量 |
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| 引用本文： | 曾锐,瞿燕,高宇明.RP-HPLC测定藏药六味能消胶囊中土木香内酯和异土木香内酯的含量[J].西南大学学报,2009,31(5). |
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| 作者姓名： | 曾锐 瞿燕 高宇明 |
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| 作者单位： | 曾锐,高宇明,ZENG Rui,GAO Yu-ming(西南民族大学,民族药物研究所,成都,610041);瞿燕,QU Yan(成都中医药大学,药学院,成都,610075) |
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| 基金项目： | 四川省科技支撑计划资助项目 |
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| 摘 要： | 目的:建立六味能消胶囊中土木香内酯和异土木香内酯的高效液相色谱法含量测定方法.方法:色谱柱为Kromasil C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸溶液(58∶42);流速为1.0 mL/min;检测波长:194 nm.结果:土木香内酯、异土木香内酯均在0.30～1.50μg范围内呈良好的线性关系;r均为0.999 8;平均回收率分别为98.2%(RSD=1.67%,n=5)和97.7%(RSD=1.48%,n=5).结论:该方法简便、灵敏、准确,可作为六味能消胶囊的质量控制方法.Abstract：Objective To establish the HPLC method for the determination of alantolactone and isoalantolactone in Liuwei Nengxiao Capsule. Method ODS column (250 mm×4. 6 mm, 5 μm) of Kromasil; the mobile phase containing a mixture of acetonitril -0.05％ phosphoric acid solution (58 : 42); flow rate 1.0 mL·min~(-1); and the wavelength as 194 nm, were utilized for this study. Results Alantolactone and isoalantolactone showed good correlation in the ranges of 0. 30～1.5 μg (r=0. 999 8). The average recovery was 98.2% for alantolactone (RSD=1. 67%, n=5) and 97.7% for isoalantolactone (RSD=1. 48%, n=5). Conclusion The method is simple, accurate and sensitive and can be used to control the quality of Liuwei Nengxiao Capsule in its production.
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| 关 键 词： | 高效液相色谱法 土木香内酯 异土木香内酯 六味能消胶囊 |
Determination of Alantolactone and Isoalantolactone in the Tibetan Madicine Liuwei Nengxiao Capsule by RP-HPLC |
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| ZENG Rui,QU Yan,GAO Yu-ming.Determination of Alantolactone and Isoalantolactone in the Tibetan Madicine Liuwei Nengxiao Capsule by RP-HPLC[J].Journal of Southwest Agricultural University,2009,31(5). |
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| Authors: | ZENG Rui QU Yan GAO Yu-ming |
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