| 木那格葡萄的组培快繁试验 |
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| 引用本文： | 高疆生,张卫芳,欧勇慧.木那格葡萄的组培快繁试验[J].落叶果树,2003,35(4):4-6. |
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| 作者姓名： | 高疆生 张卫芳 欧勇慧 |
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| 作者单位： | 1. 塔里木农垦大学植物科技学院,新疆,阿拉尔,843300
2. 新疆建设兵团农二师三十六团,若羌 |
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| 摘 要： | 以木那格葡萄的茎尖、单芽茎段为外植体，进行组培快繁试验。蛄果看出，适宜茎尖分化的培养基为B5 BA1．0mg／L IBA 0．1mg／L。适宜单芽茎段一次成苗生长的培养基为B3 BA0．05mg/L IBA0．2mg／L。适宜的生根培齐基为1／2MS IBA 0．2mg／L 蔗糖40g／L。健壮生根的试管苗要待4片叶以上移栽，移栽基质以蛭石较好。时间宜在4—5月份。
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| 关 键 词： | 木那格葡萄 组培快繁 组织培养 培养基 茎尖 单芽茎段 |
| 文章编号： | 1002-2910(2003)04-0004-03 |
| 修稿时间： | 2003年4月25日 |
Trial on rapid propagation of ‘ Munage' grape via tissue culture |
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| GAO Jiangsheng ,ZHANG Weifang ,OU Yonghui.Trial on rapid propagation of ‘ Munage'' grape via tissue culture[J].Deciduous Fruits,2003,35(4):4-6. |
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| Authors: | GAO Jiangsheng ZHANG Weifang OU Yonghui |
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| Institution: | GAO Jiangsheng 1,ZHANG Weifang 1,OU Yonghui 2 |
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| Abstract: | Rapid in vitro culture with shoot tips and single-bud segments of 'Munage'grape showed that the optimum medium for differentiation of shoot tips was B_5+BA 1.0mg/L +IBA 0.1mg/L, the optimum medium for single-bud segment to grow a seedling was B_5+BA 0.05mg/L+IBA 0.2mg/L, and the optimum rooting medium was 1/2MS+IBA 0.2mg/L+sucrose 40g/L. Wellfed shoots with 4 true leaves on each were transplanted onto vermiculite medium, with the optimum time being in April or May. |
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| Keywords: | grape tissue culture optimum medium |
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