| 提高黄色短杆菌C-11电转化效率的方法 |
| |
| 引用本文： | 谢玉清,罗淑萍,崔春生.提高黄色短杆菌C-11电转化效率的方法[J].新疆农业科学,2005,42(6):430-431. |
| |
| 作者姓名： | 谢玉清 罗淑萍 崔春生 |
| |
| 作者单位： | 新疆农科院微生物研究所,新疆乌鲁木齐,830000;新疆农业大学农学院,新疆乌鲁木齐,830052 |
| |
| 基金项目： | 国家“十五”攻关重点项目（2001BA708B02-03） |
| |
| 摘 要： | 黄色短杆菌C-11是L-丝氨酸工业化生产的优良菌株,而基因工程菌的构建是提高L-丝氨酸产量的有效途径.在基因工程菌株的构建过程中,重组质粒的转化效率是获得重组菌株的关键.电转化是近几年来被广泛应用的一种快捷而高效的转化方法.电转化效率的高低取决于菌株的特性、质粒的大小、转化的电压、温度、溶液体系、菌体与DNA的浓度等诸多因素,从几个方面就黄色短杆菌C-11电转化效率的提高方法加以论述,也为其它黄色短杆菌电转化效率的提高提供参考方法.
|
| 关 键 词： | 黄色短杆菌C-11 提高电转化效率 方法 |
| 文章编号： | 1001-4330（2005）06-0430-02 |
| 收稿时间： | 2005-05-31 |
| 修稿时间： | 2005年5月31日 |
Methods of increasing efficiency of electrotransformation of B.flavum C-11 |
| |
| XIE Yu-qing,LUO Shu-ping,CUI Chun-sheng.Methods of increasing efficiency of electrotransformation of B.flavum C-11[J].Xinjiang Agricultural Sciences,2005,42(6):430-431. |
| |
| Authors: | XIE Yu-qing LUO Shu-ping CUI Chun-sheng |
| |
| Institution: | 1. Institute of Microbiology, Xinjiang Agricultural Academy of Sciences, Urumqi 830000, China ; 2. College of Agronomy, Xinjinag Agricultural University, Urumqi 830052, China |
| |
| Abstract: | B.flavum C-11 is one of eminent strains producing L-serine,and gene-engineering strain is effective to increase the production of L-serine.The transformation efficiency is critical in the course of constructing gene-engineering strains.Electrotransformation is a quick and effective method used in recent years.The transformation efficiency is decided by characteristic of strains,size of plasmid,voltage,temperature,solution,concentration of strains and plasmids,and so on.Several ways in increasing electrotransformation efficiency as reference for other B.flavum in this essay were discussed. |
| |
| Keywords: | B flavum C-11 increase transformation efficiency method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《新疆农业科学》浏览原始摘要信息 |
| 点击此处可从《新疆农业科学》下载免费的PDF全文 |
|
