| 嗜盐菌HBCC-3的培养特性及系统发育学分析 |
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| 引用本文： | 温洪宇,韩宝平,周玉楼,吕爱军,杨柳.嗜盐菌HBCC-3的培养特性及系统发育学分析[J].安徽农业科学,2008,36(14):5804-5806. |
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| 作者姓名： | 温洪宇 韩宝平 周玉楼 吕爱军 杨柳 |
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| 作者单位： | 1. 中国矿业大学环境与测绘学院,江苏徐州,221008;徐州师范大学生命科学学院,江苏徐州,221116
2. 徐州师范大学生命科学学院,江苏徐州,221116 |
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| 基金项目： | 江苏省高校自然科学基础研究项目(06KJDl80180);徐州师范大学培育课题(06DYL03)资助. |
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| 摘 要： | 目的]为嗜盐菌HBCC-3的应用研究奠定基础。方法]从连云港台南盐场分离出嗜盐菌HBCC-3,通过正交试验对其培养条件进行优化。通过PCR方法扩增HBCC-3的16SrDNA,对其进行同源性比较和系统发育学分析。结果]嗜盐菌HBCC-3为乳白色,杆状,革兰氏染色呈阳性,菌落大小为4～6 mm。温度对HBCC-3生长影响最大,其次是摇床转速、NaCl、柠檬酸三钠、MgSO4.7H2O、pH值,而KCl最小。HBCC-3的最适培养条件为:温度35℃、pH值9.0、摇床转速200 r/min、柠檬酸三钠2.0g/L、NaCl 10%、KCl 1.0g/L、MgSO4.7H2O 10.0 g/L。菌株HBCC-3与碱杆菌属菌株Alkalibacillus salilacus BH165的相似性最高,达99%。结论]菌株HBCC-3与Alkalibacillus salilacus BH165为同一物种
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| 关 键 词： | 嗜盐菌 正交试验 16SrDNA 系统发育树 |
| 文章编号： | 0517-6611(2008)14-05804-03 |
| 修稿时间： | 2008年3月17日 |
Culture Condition Optimization of Halophilic Bacteria HBCC-3 and Its Phylogenetic Analysis |
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| WEN Hong-yu.Culture Condition Optimization of Halophilic Bacteria HBCC-3 and Its Phylogenetic Analysis[J].Journal of Anhui Agricultural Sciences,2008,36(14):5804-5806. |
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| Authors: | WEN Hong-yu |
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| Abstract: | Objective] The aim of the research was to lay the foundation for the application research of halophilic bacteria HBCC-3.Method] Halophilic bacteria HBCC-3 was isolated from Tainan salt field of Lianyungang and its culture conditions were optimized in the orthogonal test.16S rDNA of HBCC-3 was amplified by using PCR method to make the homology comparison and the phylogenetic analysis.Result] Halophilic bacteria HBCC-3 was ivory white,being rod-shaped,showing Gram-positive,with the size of bacteria colony being 4-6 mm.The effect of temperature on the growth of HBCC-3 was greatest,followed by shaker rotating speed,NaCl,trisodium citrate,MgSO4·7H2O and pH value,and that of KCl was smallest.The optimum culture conditions of HBCC-3 were as follows: the temperature of 35℃,pH value of 9.0,the shaker rotating speed of 200 r/min,trisodium citrate 2.0g/L,NaCl 10%,KCl 1.0 g/L,MgSO4·7H2O 10.0 g/L.The similarity between Strain HBCC-3 and Strain Alkalibacillus salilacus BH165 of Alkalibacillus was highest,reaching 99%.Conclusion] Strain HBCC-3 and Alkalibacillus salilacus BH165 was the same species. |
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| Keywords: | Halophilic bacteria Orthogonal test 16S rDNA Phylogenetic tree |
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